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9 Apr 2012

Volume 100, Issue 15, Articles (15xxxx)

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Appl. Phys. Lett. 100, 153701 (2012); http://dx.doi.org/10.1063/1.3700446 (3 pages)

Hsiao-lu D. Lee, Steffen J. Sahl, Matthew D. Lew, and W. E. Moerner
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The double-helix microscope super-resolves extended biological structures by localizing single blinking molecules in three dimensions with nanoscale precision

Hsiao-lu D. Lee, Steffen J. Sahl, Matthew D. Lew, and W. E. Moerner

Appl. Phys. Lett. 100, 153701 (2012); http://dx.doi.org/10.1063/1.3700446 (3 pages) | Cited 3 times

Online Publication Date: 9 April 2012

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The double-helix point spread function microscope encodes the axial (z) position information of single emitters in wide-field (x,y) images, thus enabling localization in three dimensions (3D) inside extended volumes. We experimentally determine the statistical localization precision σ of this approach using single emitters in a cell under typical background conditions, demonstrating σ < 20 nm laterally and <30 nm axially for N ≈ 1180 photons per localization. Combined with light-induced blinking of single-molecule labels, we present proof-of-concept imaging beyond the optical diffraction limit of microtubule network structures in fixed mammalian cells over a large axial range in three dimensions.
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87.64.M- Optical microscopy
87.15.-v Biomolecules: structure and physical properties
87.16.-b Subcellular structure and processes

Optical trapping force reduction and manipulation of nanoporous beads

Tao Wang, Fan Jiang, Stefan Oehrlein, Erliang Zeng, Ryan Kershner, and Franco Cerrina

Appl. Phys. Lett. 100, 153702 (2012); http://dx.doi.org/10.1063/1.3703604 (3 pages)

Online Publication Date: 11 April 2012

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We studied the interaction of infrared optical traps with controlled-pore glass (CPG) beads in aqueous medium. The lateral optical trapping force and stiffness were experimentally found considerably smaller than those of their solid counterparts. The simulation using an average refractive index revealed significant losses of effective trapping efficiency, which quantitatively agreed well with experimentally fitted curves. This effect was ascribed to the reduced relative refractive index of medium-filled CPG beads with respect to the medium. Combining optical trapping with mechanical confinements, we demonstrated a microfluidic platform allowing for the synthesis of multiple DNA oligonucleotide sequences on individual beads of interest.
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42.79.-e Optical elements, devices, and systems
47.85.Np Fluidics
42.50.Wk Mechanical effects of light on material media, microstructures and particles
42.25.-p Wave optics
42.70.Ce Glasses, quartz
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