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14 May 2012

Volume 100, Issue 20, Articles (20xxxx)

Issue Cover Spotlight Figure

Appl. Phys. Lett. 100, 203104 (2012); http://dx.doi.org/10.1063/1.3701731 (4 pages)

Z. Y. Jiang, X. X. Jiang, S. Su, X. P. Wei, S. T. Lee, and Y. He
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Two-photon fluorescence properties of curcumin as a biocompatible marker for confocal imaging

Abhishek Kumar, Lian Li, Akanksha Chaturvedi, Joseph Brzostowski, Joshna Chittigori, Susan Pierce, Lynne A. Samuelson, Daniel Sandman, and Jayant Kumar

Appl. Phys. Lett. 100, 203701 (2012); http://dx.doi.org/10.1063/1.4717753 (4 pages) | Cited 2 times

Online Publication Date: 15 May 2012

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Two-photon (TP) fluorescence properties of an antioxidant and anti-tumor molecule, curcumin, were investigated. The two-photon absorption (TPA) action cross-section was measured in organic solvents and found to be 6 GM in tetrahydrofuran and 2 GM in dimethyl sulfoxide. The measured TPA cross-section is comparable to that of rhodamine 6G. One-photon and TP confocal microscopy has demonstrated that curcumin is internalized in cells and can be used for imaging applications. Our investigation indicates that curcumin is a viable biocompatible TP fluorescent marker.
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87.15.mq Luminescence
33.50.Dq Fluorescence and phosphorescence spectra

Collective sub-diffusive dynamics in bacterial carpet microfluidic channel

Yi-Teng Hsiao, Jing-Hui Wang, Yi-Chun Hsu, Chien-Chun Chiu, Chien-Jung Lo, Chia-Wen Tsao, and Wei Yen Woon

Appl. Phys. Lett. 100, 203702 (2012); http://dx.doi.org/10.1063/1.4720072 (3 pages) | Cited 1 time

Online Publication Date: 17 May 2012

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We experimentally investigate the collective dynamics in bacterial carpet microfluidic channel. The microfluidic channel is composed of single polar flagellated Vibrio alginolyticus deposited glass substrates. The individual flagellum swimming speed is tuned by varying buffer sodium concentration. Hydrodynamic coupling strength is tuned by varying buffer viscosity. The attached bacteria constantly perform two major modes in flagellum motion, namely, the local rotation and large angle flick. Particle tracking statistics shows high flagellum rotational rate and strong hydrodynamic coupling strength lead to collective sub-diffusive dynamics. The observed effect is strongly correlated to hydrodynamic coupling of flick motions between nearby bacteria.
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87.80.Ek Mechanical and micromechanical techniques
07.10.Cm Micromechanical devices and systems
85.85.+j Micro- and nano-electromechanical systems (MEMS/NEMS) and devices
87.17.Rt Cell adhesion and cell mechanics
87.17.Uv Biotechnology of cell processes

Near-field ablation threshold of cellular samples in the mid-infrared wavelength region

Deepa Raghu, Joan A. Hoffmann, Benjamin Gamari, and M. E. Reeves

Appl. Phys. Lett. 100, 203703 (2012); http://dx.doi.org/10.1063/1.4719043 (5 pages) | Cited 1 time

Online Publication Date: 18 May 2012

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We report the near-field ablation of biological material with spot sizes as small as 1.5 μm under 3 μm wavelength radiation. The fluence dependence of the ablation of both cellulose acetate coverslips in water and myoblast cell samples in growth media has been studied. We find that for all near-field experiments, the ablation thresholds are very high compared to far-field experiments. A detailed analysis of the length and time scales of the systems provides support for the identification of a plasma-induced mechanism. Thus, applications of sub-wavelength ablation will require robust near-field techniques with capability for high-power density delivery of light.
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87.17.Ee Growth and division
87.50.W- Optical/infrared radiation effects
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