APL Nameplate
  • Volume/Page
  • Keyword
  • DOI
  • Citation
  • Advanced
   
 
 
 

Flickr Twitter iResearch App Facebook

BROWSE VOLUMES

Year Range: 
Search Issue | RSS Feeds RSS
Previous Issue Next Issue

20 Feb 2012

Volume 100, Issue 8, Articles (08xxxx)

Issue Cover Spotlight Figure

Appl. Phys. Lett. 100, 082401 (2012); http://dx.doi.org/10.1063/1.3684972 (4 pages)

Elizabeth Rapoport and Geoffrey S. D. Beach
Enlarge Image | Read More
Return to All Sections
back to top
RSS Feeds

DNA-cisplatin binding mechanism peculiarities studied with single molecule stretching experiments

F. A. P. Crisafuli, E. C. Cesconetto, E. B. Ramos, and M. S. Rocha

Appl. Phys. Lett. 100, 083701 (2012); http://dx.doi.org/10.1063/1.3689774 (3 pages) | Cited 1 time

Online Publication Date: 24 February 2012

Full Text: Read Online (HTML) | Download PDF

Show Abstract
We propose a method to determine the DNA-cisplatin binding mechanism peculiarities by monitoring the mechanical properties of these complexes. To accomplish this task, we have performed single molecule stretching experiments by using optical tweezers, from which the persistence and contour lengths of the complexes can be promptly measured. The persistence length of the complexes as a function of the drug total concentration in the sample was used to deduce the binding data, from which we show that cisplatin binds cooperatively to the DNA molecule, a point which so far has not been stressed in binding equilibrium studies of this ligand.
Show PACS
87.80.Cc Optical trapping
87.14.gk DNA
87.15.B- Structure of biomolecules
87.15.La Mechanical properties
Return to All Sections
Close

Google Calendar

ADVERTISEMENT

Go to AIP Home   © AIP Publishing LLC
close