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10 Sep 2012

Volume 101, Issue 11, Articles (11xxxx)

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Appl. Phys. Lett. 101, 113302 (2012); http://dx.doi.org/10.1063/1.4749791 (4 pages)

Chang-Hoon Shim, Shuzo Hirata, Juro Oshima, Tomohiko Edura, Reiji Hattori, and Chihaya Adachi
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Fiber-optic Raman sensing of cell proliferation probes and molecular vibrations: Brain-imaging perspective

Lyubov V. Doronina-Amitonova, Il'ya V. Fedotov, Olga I. Ivashkina, Marina A. Zots, Andrei B. Fedotov, Konstantin V. Anokhin, and Aleksei M. Zheltikov

Appl. Phys. Lett. 101, 113701 (2012); http://dx.doi.org/10.1063/1.4751468 (3 pages)

Online Publication Date: 10 September 2012

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Optical fibers are employed to sense fingerprint molecular vibrations in ex vivo experiments on the whole brain and detect cell proliferation probes in a model study on a quantitatively controlled solution. A specifically adapted spectral filtering procedure is shown to allow the Raman signal from molecular vibrations of interest to be discriminated against the background from the fiber, allowing a highly sensitive Raman detection of the recently demonstrated EdU (5-ethynyl-2′-deoxyuridine) labels of DNA synthesis in cells.
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87.63.L- Visual imaging
87.16.-b Subcellular structure and processes
42.81.Pa Sensors, gyros

Ultra-high cooling rate utilizing thin film evaporation

Fengmin Su, Hongbin Ma, Xu Han, Hsiu-hung Chen, and Bohan Tian

Appl. Phys. Lett. 101, 113702 (2012); http://dx.doi.org/10.1063/1.4752253 (3 pages)

Online Publication Date: 10 September 2012

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This research introduces a cell cryopreservation method, which utilizes thin film evaporation and provides an ultra-high cooling rate. The microstructured surface forming the thin film evaporation was fabricated from copper microparticles with an average diameter of 50 μm. Experimental results showed that a cooling rate of approximately 5×104 °C/min was achieved in a temperature range from 10 °C to −187 °C. The current investigation will give birth to a cell cryopreservation method through vitrification with relatively low concentrations of cryoprotectants.
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68.55.-a Thin film structure and morphology
68.43.Mn Adsorption kinetics
68.35.B- Structure of clean surfaces (and surface reconstruction)
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