APL Nameplate
  • Volume/Page
  • Keyword
  • DOI
  • Citation
  • Advanced
   
 
 
 

Flickr Twitter iResearch App Facebook

BROWSE VOLUMES

Year Range: 
Search Issue | RSS Feeds RSS
Previous Issue Next Issue

12 Nov 2012

Volume 101, Issue 20, Articles (20xxxx)

Issue Cover Spotlight Figure

Appl. Phys. Lett. 101, 203102 (2012); http://dx.doi.org/10.1063/1.4747717 (4 pages)

Hyukjin Jung and Ki-Hun Jeong
Enlarge Image | Read More
Return to All Sections
back to top
RSS Feeds

Quantitative phase imaging of human red blood cells using phase-shifting white light interference microscopy with colour fringe analysis

Dalip Singh Mehta and Vishal Srivastava

Appl. Phys. Lett. 101, 203701 (2012); http://dx.doi.org/10.1063/1.4767519 (5 pages) | Cited 1 time

Online Publication Date: 12 November 2012

Full Text: Read Online (HTML) | Download PDF

Show Abstract
We report quantitative phase imaging of human red blood cells (RBCs) using phase-shifting interference microscopy. Five phase-shifted white light interferograms are recorded using colour charge coupled device camera. White light interferograms were decomposed into red, green, and blue colour components. The phase-shifted interferograms of each colour were then processed by phase-shifting analysis and phase maps for red, green, and blue colours were reconstructed. Wavelength dependent refractive index profiles of RBCs were computed from the single set of white light interferogram. The present technique has great potential for non-invasive determination of refractive index variation and morphological features of cells and tissues.
Show PACS
87.57.nf Reconstruction
87.64.M- Optical microscopy
42.79.Pw Imaging detectors and sensors
42.87.Bg Phase shifting interferometry
87.16.-b Subcellular structure and processes

Dynamics of cellular response to hypotonic stimulation revealed by quantitative phase microscopy and multi-fractal detrended fluctuation analysis

Nelson Cardenas, Satish Kumar, and Samarendra Mohanty

Appl. Phys. Lett. 101, 203702 (2012); http://dx.doi.org/10.1063/1.4766922 (4 pages) | Cited 1 time

Online Publication Date: 13 November 2012

Full Text: Read Online (HTML) | Download PDF

Show Abstract
Hypotonic stimulation is known to cause morphological changes in cells and also leads to modulation of cellular physiology. In order to evaluate the dynamics of cellular response to hypotonic stimulation, we utilized digital holographic microscopy for quantitative phase microscopy, achieved by a common-path interferometry geometry based on extraction of reference beam by spatial-filtering. Results from live cell investigations demonstrate the capability of this method for dynamic quantitative phase imaging. Further, wavelet and multi-fractal detrended fluctuation analysis revealed that the dynamic phase changes, in response to hypotonic stimulation, are multifractal in nature.
Show PACS
87.17.Pq Morphogenesis
87.64.M- Optical microscopy
02.30.Uu Integral transforms
05.40.-a Fluctuation phenomena, random processes, noise, and Brownian motion
05.45.Df Fractals
42.40.My Applications
Return to All Sections
Close

Google Calendar

ADVERTISEMENT

Go to AIP Home   © AIP Publishing LLC
close