APL Nameplate
  • Volume/Page
  • Keyword
  • DOI
  • Citation
  • Advanced
   
 
 
 

Flickr Twitter iResearch App Facebook

BROWSE VOLUMES

Year Range: 
Search Issue | RSS Feeds RSS
Previous Issue Next Issue

3 Dec 2012

Volume 101, Issue 23, Articles (23xxxx)

Issue Cover Spotlight Figure

Appl. Phys. Lett. 101, 233101 (2012); http://dx.doi.org/10.1063/1.4749281 (3 pages)

S. A. Studenikin, J. Thorgrimson, G. C. Aers, A. Kam, P. Zawadzki, Z. R. Wasilewski, A. Bogan, and A. S. Sachrajda
Enlarge Image | Read More
Return to All Sections
back to top
RSS Feeds

Nanogap-based dielectric-specific colocalization for highly sensitive surface plasmon resonance detection of biotin-streptavidin interactions

Yonghwi Kim, Kyungwha Chung, Wonju Lee, Dong Ha Kim, and Donghyun Kim

Appl. Phys. Lett. 101, 233701 (2012); http://dx.doi.org/10.1063/1.4769108 (5 pages)

Online Publication Date: 3 December 2012

Full Text: Read Online (HTML) | Download PDF

Show Abstract
We have performed highly sensitive surface plasmon resonance (SPR) detection by colocalizing the evanescent near-fields and target molecular distribution. The colocalization is based on oblique metal evaporation to form nanogaps of a size under 100 nm without using electron-beam lithography. The concept was demonstrated by detecting siloxane-based biotin/streptavidin interactions. 50-nm nanogaps produced the largest amplification of optical signatures and two orders of magnitude enhancement of sensitivity over conventional thin film-based measurements. The enhancement is associated with efficient overlap of localized near-fields and target. Colocalized detection scheme is expected to provide clues to molecular sensitivity for SPR biosensing.
Show PACS
87.15.hg Dynamics of intermolecular interactions
87.80.-y Biophysical techniques (research methods)
73.21.-b Electron states and collective excitations in multilayers, quantum wells, mesoscopic, and nanoscale systems

Multicolor in vivo brain imaging with a microscope-coupled fiber-bundle microprobe

Lyubov V. Doronina-Amitonova, Il'ya V. Fedotov, Olga Efimova, Maria Chernysheva, Andrei B. Fedotov, Konstantin V. Anokhin, and Aleksei M. Zheltikov

Appl. Phys. Lett. 101, 233702 (2012); http://dx.doi.org/10.1063/1.4767386 (3 pages) | Cited 1 time

Online Publication Date: 4 December 2012

Full Text: Read Online (HTML) | Download PDF

Show Abstract
A fiber-bundle microprobe coupled to a confocal optical microscope is shown to enable multicolor in vivo fluorescence brain imaging. A bundle of several thousands of 2.4-μm-diameter optical fibers is employed to deliver multiwavelength laser excitation radiation and to transmit multicolor images from hippocampus tissues in living transgenic mice by picking up a multiplex fluorescent response from green fluorescent protein, nucleic acid counterstains, and neuron tracers.
Show PACS
87.85.Pq Biomedical imaging
87.17.-d Cell processes
87.64.M- Optical microscopy

Fluorescence resonance energy transfer-based molecular logic circuit using a DNA scaffold

Takahiro Nishimura, Yusuke Ogura, and Jun Tanida

Appl. Phys. Lett. 101, 233703 (2012); http://dx.doi.org/10.1063/1.4769812 (4 pages)

Online Publication Date: 6 December 2012

Full Text: Read Online (HTML) | Download PDF

Show Abstract
This paper presents a method of information processing using biomolecular input signals and fluorescence resonance energy transfer (FRET) signaling constructed on a DNA scaffold. Logic operations are achieved by encoding molecular inputs into an arrangement of fluorescence dyes using simple DNA reactions and by evaluating a logic expression using local photonic signaling that is much faster than DNA reactions. Experimental results verify the operation of a complete set of Boolean logic functions (AND, OR, NOT) and combinational logic operations using a FRET-signal cascade.
Show PACS
87.15.mq Luminescence
02.10.-v Logic, set theory, and algebra
02.30.-f Function theory, analysis
84.30.Sk Pulse and digital circuits
85.65.+h Molecular electronic devices
87.14.gk DNA
87.15.B- Structure of biomolecules
87.15.H- Dynamics of biomolecules

Effects of water molecules on binding kinetics of peptide receptor on a piezoelectric microcantilever

Sang Hui Kim, Yong Kyoung Yoo, Myung-Sic Chae, Ji Yoon Kang, Tae Song Kim, Kyo Seon Hwang, and Jeong Hoon Lee

Appl. Phys. Lett. 101, 233704 (2012); http://dx.doi.org/10.1063/1.4769969 (4 pages)

Online Publication Date: 6 December 2012

Full Text: Read Online (HTML) | Download PDF

Show Abstract
The use of highly selective reversible peptide receptors is essential for cantilever-based electronic nose systems. Here, we present the effects of water molecules on the binding kinetics of 2,4-dinitrotoluene (DNT) molecules with DNT selective peptide receptors linked with a tri(ethylene glycol)-based (TEG) self-assembled monolayer (SAM) in a gas phase in a piezoelectric microcantilever sensor. We observed 1.5-times faster reaction kinetics in wet conditions compared with dry conditions. In a dissociation step, distinctive differences in the recovery time were observed in wet conditions, which could be attributed to water retention efficiency of TEG-linkers for the conformation of biomolecules.
Show PACS
87.80.Ek Mechanical and micromechanical techniques
82.39.-k Chemical kinetics in biological systems
82.80.-d Chemical analysis and related physical methods of analysis
87.14.ef Peptides
87.15.hp Conformational changes
87.15.rs Dissociation

Combinatorial matrix-assisted pulsed laser evaporation: Single-step synthesis of biopolymer compositional gradient thin film assemblies

F. Sima, E. Axente, L. E. Sima, U. Tuyel, M. S. Eroglu, N. Serban, C. Ristoscu, S. M. Petrescu, E. Toksoy Oner, and I. N. Mihailescu

Appl. Phys. Lett. 101, 233705 (2012); http://dx.doi.org/10.1063/1.4769987 (5 pages)

Online Publication Date: 7 December 2012

Full Text: Read Online (HTML) | Download PDF

Show Abstract
We introduce a combinatorial approach for the fabrication of organic biopolymer thin films. Structures with compositional gradient are obtained by simultaneous laser vaporization of two distinct targets. Matrix-assisted pulsed laser evaporation deposition method was applied to obtain a compositional library of levan and oxidized levan in form of thin film. The gradient of film composition and structure was demonstrated by infrared spectroscopy while in vitro cell culture assays illustrated characteristic responses of cells to specific surface regions. The method can rapidly generate discrete areas of organic film compositions with improved properties than starting materials.
Show PACS
81.15.Fg Pulsed laser ablation deposition
68.55.am Polymers and organics
81.15.Dj E-beam and hot filament evaporation deposition
61.41.+e Polymers, elastomers, and plastics
78.35.+c Brillouin and Rayleigh scattering; other light scattering
78.66.Qn Polymers; organic compounds
Return to All Sections
Close

Google Calendar

ADVERTISEMENT

Go to AIP Home   © AIP Publishing LLC
close