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30 Jul 2012

Volume 101, Issue 5, Articles (05xxxx)

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Appl. Phys. Lett. 101, 051103 (2012); http://dx.doi.org/10.1063/1.4738774 (4 pages)

Alec Rose, Da Huang, and David R. Smith
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Detection of sugar-lectin interactions by multivalent dendritic sugar functionalized single-walled carbon nanotubes

K. S. Vasu, K. Naresh, R. S. Bagul, N. Jayaraman, and A. K. Sood

Appl. Phys. Lett. 101, 053701 (2012); http://dx.doi.org/10.1063/1.4739793 (4 pages)

Online Publication Date: 30 July 2012

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We show that single walled carbon nanotubes (SWNTs) decorated with sugar functionalized poly (propyl ether imine) (PETIM) dendrimer is a very sensitive platform to quantitatively detect carbohydrate recognizing proteins, namely, lectins. The changes in electrical conductivity of SWNT in field effect transistor device due to carbohydrate–protein interactions form the basis of present study. The mannose sugar attached PETIM dendrimers undergo charge–transfer interactions with the SWNTs. The changes in the conductance of the dendritic sugar functionalized SWNT after addition of lectins in varying concentrations were found to follow the Langmuir type isotherm, giving the concanavalin A (Con A)-mannose affinity constant to be 8.5 × 106 M−1. The increase in the device conductance observed after adding 10 nM of Con A is same as after adding 20 μM of a non-specific lectin peanut agglutinin, showing the high specificity of the Con A–mannose interactions. The specificity of sugar-lectin interactions was characterized further by observing significant shifts in Raman modes of the SWNTs.
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87.15.K- Molecular interactions; membrane-protein interactions
85.65.+h Molecular electronic devices
87.80.-y Biophysical techniques (research methods)
87.14.E- Proteins

Nonlinear electro-mechanobiological behavior of cell membrane during electroporation

Peigang Deng, Yi-Kuen Lee, Ran Lin, and Tong-Yi Zhang

Appl. Phys. Lett. 101, 053702 (2012); http://dx.doi.org/10.1063/1.4739940 (4 pages)

Online Publication Date: 30 July 2012

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A nonlinear electroporation (EP) model is proposed to study the electro-mechanobiological behavior of cell membrane during EP, by taking the nonlinear large deformation of the membrane into account. The proposed model predicts the critical transmembrane potential and the activation energy for EP, the equilibrium pore size, and the resealing process of the pore. Single-cell EP experiments using a micro EP chip were conducted on chicken red blood cells at different temperatures to determine the activation energy and the critical transmembrane potential for EP. The experimental results are in good agreement with the theoretical predictions.
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87.50.cj Electroporation/membrane effects
87.16.dm Mechanical properties and rheology

Reactive oxygen species-related plasma effects on the apoptosis of human bladder cancer cells in atmospheric pressure pulsed plasma jets

Hea Min Joh, Sun Ja Kim, T. H. Chung, and S. H. Leem

Appl. Phys. Lett. 101, 053703 (2012); http://dx.doi.org/10.1063/1.4742742 (5 pages) | Cited 2 times

Online Publication Date: 3 August 2012

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Helium/oxygen atmospheric pressure plasma jets driven by pulsed dc voltage with repetition rate of several tens of kilohertz are utilized for plasma-cell interactions. The effect of operating parameters on the apoptosis of cultured human bladder (EJ) cancer cells is investigated. The parameters such as applied voltage, pulse repetition frequency, and duty ratio determine the plasma dose. The apoptotic changes in cells with plasma treatment are detected by staining assay and flow cytometry. Apoptosis rates are observed to correlate well with both the plasma dose and the levels of intracellular and extracellular reactive oxygen species.
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87.55.dk Dose-volume analysis
52.40.-w Plasma interactions (nonlaser)
52.75.-d Plasma devices
87.17.-d Cell processes
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