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9 Apr 2001

Volume 78, Issue 15, pp. 2095-2255

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Nonoptically probing near-field microscopy for the observation of biological living specimens

Yoshimasa Kawata, Manabu Murakami, Chikara Egami, Okihiro Sugihara, Naomichi Okamoto, Masaaki Tsuchimori, Osamu Watanabe, and Osamu Nakamura

Appl. Phys. Lett. 78, 2247 (2001); http://dx.doi.org/10.1063/1.1350965 (3 pages) | Cited 8 times

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We present the observation of living specimens with subwavelength resolution by using the nonoptically probing near-field microscopy we have developed recently. In the near-field microscope, the optical field distributions near the specimens are recorded as the surface topography of a photosensitive film, and the topographical distributions are readout with an atomic-force microscopy. Since the near-field microscope does not require the scanning of a probe tip for illumination or detection or scattering of light, it is possible to observe moving biological specimens and fast phenomena. We demonstrate the observation of a moving paramecium and euglena gracilis with subwavelength resolution. The observation of the nucleus inside a euglena cell was also demonstrated. © 2001 American Institute of Physics.
Show PACS
87.80.-y Biophysical techniques (research methods)
87.16.Tb Mitochondria and other organelles
07.79.Fc Near-field scanning optical microscopes
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