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16 Apr 2001

Volume 78, Issue 16, pp. 2267-2404

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Ion-beam-induced deoxyribose nucleic acid transfer

S. Anuntalabhochai, R. Chandej, B. Phanchaisri, L. D. Yu, T. Vilaithong, and I. G. Brown

Appl. Phys. Lett. 78, 2393 (2001); http://dx.doi.org/10.1063/1.1362338 (3 pages) | Cited 20 times

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We report our observations of the interaction of energetic ions with bacterial cells, inducing direct deoxyribose nucleic acid (DNA) transfer into Escherichia coli (E. coli). Argon- and nitrogen-ion beams were used to bombard the bacteria E. coli in a vacuum with energy of 26 keV and fluence in the range 0.5–4×1015 ions/cm2. Three DNA plasmids, pGEM2, pGEM-T easy, and pGFP, carrying different marker genes, were subsequently transferred (separately) into the appropriately ion-bombarded bacteria and successfully expressed. The results of this study indicate that ion beams with an energy such that the ion range is approximately equal to the cell envelope thickness, at a certain range of fluence, are able to generate pathways for macromolecule transfer through the envelope without irreversible damage. © 2001 American Institute of Physics.
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87.53.-j Effects of ionizing radiation on biological systems
87.14.G- Nucleic acids
87.15.M- Spectra of biomolecules
87.16.-b Subcellular structure and processes

Ordered stretching of single molecules of deoxyribose nucleic acid between microfabricated polystyrene lines

D. C. G. Klein, L. Gurevich, J. W. Janssen, L. P. Kouwenhoven, J. D. Carbeck, and L. L. Sohn

Appl. Phys. Lett. 78, 2396 (2001); http://dx.doi.org/10.1063/1.1365099 (3 pages) | Cited 14 times

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A technique for creating arrays of parallel, stretched single molecules of deoxyribose nucleic acid (DNA) on an arbitrary substrate for high-resolution scanning-probe imaging is discussed. The technique consists of lithographically patterning polystyrene lines on a substrate which then provide attachment sites for the ends of individual DNA molecules. Molecular combing is performed to stretch DNA from one polystyrene line to the other. Scanning-tunneling and atomic-force microscope images of single molecules of bacteriophage-lambda DNA are shown to demonstrate the advantages of this technique. Several applications, from high-resolution genomics to molecular electronics, are discussed. © 2001 American Institute of Physics.
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87.14.G- Nucleic acids
07.79.-v Scanning probe microscopes and components
87.64.Dz Scanning tunneling and atomic force microscopy
87.85.Va Micromachining
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