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30 Apr 2001

Volume 78, Issue 18, pp. 2617-2804

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Sensing metabolites using donor–acceptor nanodistributions in fluorescence resonance energy transfer

O. J. Rolinski, D. J. S. Birch, L. J. McCartney, and J. C. Pickup

Appl. Phys. Lett. 78, 2796 (2001); http://dx.doi.org/10.1063/1.1367308 (3 pages) | Cited 7 times

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Before fluorescence sensing techniques can be applied to media as delicate and complicated as human tissue, an adequate interpretation of the measured observables is required, i.e., an inverse problem needs to be solved. Recently we have solved the inverse problem relating to the kinetics of fluorescence resonance energy transfer (FRET), which clears the way for the determination of the donor–acceptor distribution function in FRET assays. In this letter this approach to monitoring metabolic processes is highlighted and the application to glucose sensing demonstrated. © 2001 American Institute of Physics.
Show PACS
07.07.Df Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing
87.80.-y Biophysical techniques (research methods)
78.55.Kz Solid organic materials
78.67.Bf Nanocrystals, nanoparticles, and nanoclusters
02.30.Zz Inverse problems
82.80.Dx Analytical methods involving electronic spectroscopy
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