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12 Feb 2001

Volume 78, Issue 7, pp. 853-1016

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Dielectric spectroscopy for bioanalysis: From 40 Hz to 26.5 GHz in a microfabricated wave guide

G. R. Facer, D. A. Notterman, and L. L. Sohn

Appl. Phys. Lett. 78, 996 (2001); http://dx.doi.org/10.1063/1.1347020 (3 pages) | Cited 37 times

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We report developing coplanar waveguide devices which can perform dielectric spectroscopy on biological samples within a microfluidic channel or well. Since coupling to the fluid sample is capacitive, no surface functionalization or chemical sample preparation are required. Data on cell suspensions and solutions of proteins and nucleic acids spanning the frequency range from 40 Hz to 26.5 GHz are presented. Low-frequency data are well explained using a simple dispersion model. At microwave frequencies, the devices yield reproducible and distinguishable spectral responses for hemoglobin solution and live E. coli. © 2001 American Institute of Physics.
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87.64.-t Spectroscopic and microscopic techniques in biophysics and medical physics
84.40.Az Waveguides, transmission lines, striplines
47.85.Np Fluidics
87.17.-d Cell processes
87.15.M- Spectra of biomolecules
87.14.E- Proteins
82.39.Pj Nucleic acids, DNA and RNA bases
82.70.Kj Emulsions and suspensions

Three-dimensional subsurface microprocessing of collagen by ultrashort laser pulses

Nicholas I. Smith, Katsumasa Fujita, Osamu Nakamura, and Satoshi Kawata

Appl. Phys. Lett. 78, 999 (2001); http://dx.doi.org/10.1063/1.1347392 (3 pages) | Cited 7 times

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Submicron sized damage spots were created by multiphoton absorption of ultrashort 775 nm laser pulses in collagen gel stained with fluorescent dye. Submicron nodules were formed in the collagen both near the surface and at a depth of up to 30 μm. We have shown that large numbers of pulses at low pulse energy provide well-controlled sample damage restricted to a small focal volume. The damage threshold was dependent on dye concentration but not significantly dependent on the depth. This will be useful for experiments that study laser-induced damage in living biological samples on a cellular scale and deep within the specimen. © 2001 American Institute of Physics.
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87.50.W- Optical/infrared radiation effects
87.14.E- Proteins
87.17.-d Cell processes
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