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20 Aug 2001

Volume 79, Issue 8, pp. 1073-1217

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Generation of calcium waves in living cells by pulsed-laser-induced photodisruption

Nicholas I. Smith, Katsumasa Fujita, Tomoyuki Kaneko, Kentaro Katoh, Osamu Nakamura, Satoshi Kawata, and Tetsuro Takamatsu

Appl. Phys. Lett. 79, 1208 (2001); http://dx.doi.org/10.1063/1.1397255 (3 pages) | Cited 25 times

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Here, we present an optical technique that can induce waves of calcium ion concentration in live biological cells. Ca2+ waves were induced by femtosecond pulsed-laser illumination. Living HeLa cells were exposed to focused 140 fs pulses of 780 nm wavelength at 30 mW average power. Ca2+ waves were imaged by fluorescence and were observed to propagate from the laser focal point inside the cell. Photoinduced generation of Ca2+ waves can be performed at any point inside the cell, an improvement over previous mechanical or biochemical stimulation techniques. © 2001 American Institute of Physics.
Show PACS
87.50.W- Optical/infrared radiation effects
87.16.Uv Active transport processes
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