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7 Oct 2002

Volume 81, Issue 15, pp. 2677-2902

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Morphological and biochemical analysis by atomic force microscopy and scanning near-field optical microscopy techniques of human keratinocytes (HaCaT) exposed to extremely low frequency 50 Hz magnetic field

Sabrina Rieti, Vanessa Manni, Antonella Lisi, Settimio Grimaldi, Renato Generosi, Marco Luce, Paolo Perfetti, Antonio Cricenti, Deleana Pozzi, and Livio Giuliani

Appl. Phys. Lett. 81, 2890 (2002); http://dx.doi.org/10.1063/1.1506011 (3 pages) | Cited 2 times

Online Publication Date: 30 September 2002

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We studied the effect of the interaction of electromagnetic radiation with human keratinocytes (HaCaT), at low (50 Hz, 1 mT) frequency using both atomic force microscopy (AFM) and scanning near-field optical microscopy (SNOM) techniques. AFM analysis showed modifications in shape and morphology in exposed cells, while SNOM indirect immunofluorescence analysis revealed an increase of segregation of β4 integrin (an adhesion marker) in the cell membrane of the same cells, suggesting that a higher percentage of the exposed cells shows a modified pattern of this adhesion marker. © 2002 American Institute of Physics.
Show PACS
87.50.C- Static and low-frequency electric and magnetic fields effects
87.17.-d Cell processes
87.64.mt Near-field scanning
87.64.Dz Scanning tunneling and atomic force microscopy
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