APL Nameplate
  • Volume/Page
  • Keyword
  • DOI
  • Citation
  • Advanced
   
 
 
 

Flickr Twitter iResearch App Facebook

BROWSE VOLUMES

Year Range: 
Search Issue | RSS Feeds RSS
Previous Issue Next Issue

23 Dec 2002

Volume 81, Issue 26, pp. 4895-5074

Return to All Sections
back to top
RSS Feeds

Observations of pressure-wave-excited contrast agent bubbles in the vicinity of cells

B. Wolfrum, R. Mettin, T. Kurz, and W. Lauterborn

Appl. Phys. Lett. 81, 5060 (2002); http://dx.doi.org/10.1063/1.1531225 (3 pages) | Cited 24 times

Online Publication Date: 17 December 2002

Full Text: Read Online (HTML) | Download PDF

Show Abstract
Levovist contrast agent bubble dynamics after spark-induced pressure wave exposure is investigated in the vicinity of normal rat kidney fibroblast cells. The bubble dynamics is calculated using the Keller–Miksis model and results are compared with the experimental radius time curves. High-speed microscopic images show rapid bubble expansion and collapse. Even at moderate peak negative pressure amplitudes of less than 2 MPa the contrast agent bubbles have been observed to expand to more than 30 times their original radius and to rupture cells upon collapse. © 2002 American Institute of Physics.
Show PACS
87.50.Y- Biological effects of acoustic and ultrasonic energy
43.80.Gx Mechanisms of action of acoustic energy on biological systems: physical processes, sites of action

Realization of hollow SiO2 micronozzles for electrical measurements on living cells

T. Lehnert, M. A. M. Gijs, R. Netzer, and U. Bischoff

Appl. Phys. Lett. 81, 5063 (2002); http://dx.doi.org/10.1063/1.1528292 (3 pages) | Cited 34 times

Online Publication Date: 17 December 2002

Full Text: Read Online (HTML) | Download PDF

Show Abstract
We present a microfluidic device for the immobilization and electrical measurements, such as patch-clamp or impedance measurements, on individual living cells. Micron-sized hollow SiO2 nozzles are realized in Si wafers using a deep plasma etching process. The micronozzles are integrated with glass wafers containing microfluidic channels and Ag/AgCl electrodes. Reliable cell positioning on the nozzles via hydrodynamic forces is obtained. Relevant electrical parameters of the system, especially seal resistances between attached cells and the nozzle, are determined. © 2002 American Institute of Physics.
Show PACS
87.80.Fe Micromanipulation of biological structures
87.80.Jg Patch clamping and other physiological measurements
87.85.Va Micromachining
87.17.-d Cell processes
47.60.-i Flow phenomena in quasi-one-dimensional systems
47.85.Np Fluidics
85.85.+j Micro- and nano-electromechanical systems (MEMS/NEMS) and devices
07.10.Cm Micromechanical devices and systems
52.77.Bn Etching and cleaning
81.65.Cf Surface cleaning, etching, patterning
Return to All Sections
Close

Google Calendar

ADVERTISEMENT

Go to AIP Home   © AIP Publishing LLC
close