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5 May 2003

Volume 82, Issue 18, pp. 2939-3130

Issue Cover Spotlight Figure

Appl. Phys. Lett. 82, 2957 (2003); http://dx.doi.org/10.1063/1.1571977 (3 pages)

Tadashi Kawazoe, Kiyoshi Kobayashi, Suguru Sangu, and Motoichi Ohtsu
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Fabrication of biological microarrays using microcantilevers

P. Belaubre, M. Guirardel, G. Garcia, J. B. Pourciel, V. Leberre, A. Dagkessamanskaia, E. Trévisiol, J. M. François, and C. Bergaud

Appl. Phys. Lett. 82, 3122 (2003); http://dx.doi.org/10.1063/1.1565685 (3 pages) | Cited 26 times

Online Publication Date: 29 April 2003

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Arrays of silicon-based microcantilevers with properly designed passivated aluminum electrodes have been used to generate microarrays by depositing microspots of biological samples using a direct contact deposition technique. The approach proposed here can be compared to the dip-pen technique but with the noticeable difference that electrostatic fields are generated onto the cantilevers to increase the height of liquid rise on the cantilever surface when dipping them into the liquid to be deposited. Both electrowetting through the reduction of the contact angle and dielectrophoresis through electrostatic forces can be used to favor the loading efficiency. These phenomena are particularly pronounced on the microscale due to the fact that physical scaling laws favor electrostatic forces. Moreover, at this scale, conductive heat dissipation is enhanced and therefore joule heating can be minimized. Using this approach, with a single loading, arrays of more than a hundred spots, from the femtoliter to the picoliter range, containing fluorescent-labeled oligonucleotides and proteins were directly patterned on a glass slide. © 2003 American Institute of Physics.
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87.80.-y Biophysical techniques (research methods)
87.14.E- Proteins
82.45.-h Electrochemistry and electrophoresis

Laser-diode-stimulated emission depletion microscopy

V. Westphal, C. M. Blanca, M. Dyba, L. Kastrup, and S. W. Hell

Appl. Phys. Lett. 82, 3125 (2003); http://dx.doi.org/10.1063/1.1571656 (3 pages) | Cited 26 times

Online Publication Date: 29 April 2003

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We report subdiffraction resolution in far-field fluorescence microscopy through laser-diode-stimulated emission depletion of molecular markers. The diode-generated focal intensities lead to a resolution improvement by ∼ 45% in both lateral directions. Excitation and stimulated emission are performed with electronically synchronized diode pulses of 50–70 ps and 300–400 ps duration, respectively. The subdiffraction resolution is utilized to resolve neighboring individual molecules. © 2003 American Institute of Physics.
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07.79.-v Scanning probe microscopes and components
42.30.Va Image forming and processing
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