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29 Sep 2003

Volume 83, Issue 13, pp. 2503-2719

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Appl. Phys. Lett. 83, 2680 (2003); http://dx.doi.org/10.1063/1.1614845 (3 pages)

F. Nakajima, Y. Miyoshi, J. Motohisa, and T. Fukui
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Suspended microchannel resonators for biomolecular detection

T. P. Burg and S. R. Manalis

Appl. Phys. Lett. 83, 2698 (2003); http://dx.doi.org/10.1063/1.1611625 (3 pages) | Cited 52 times

Online Publication Date: 23 September 2003

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We present a resonant mass sensor for specific biomolecular detection in a subnanoliter fluid volume. The sensing principle is based on measuring shifts in resonance frequency of a suspended microfluidic channel upon accumulation of molecules on the inside walls of the device. Confining the fluid to the inside of a hollow cantilever enables direct integration with conventional microfluidic systems, significantly increases sensitivity by eliminating high damping and viscous drag, and allows the resonator to be actuated by electrostatic forces. Fluid density measurements reveal a mass resolution of 10−17 g/μm2 in a 4 mHz–4 Hz bandwidth. To demonstrate biomolecular detection, we present real-time measurements of the specific binding between avidin and biotinylated bovine serum albumin. Based on these measurements, we expect that changes in surface mass loading on the order of 10−19 g/μm2 can be detected in an optimized system. © 2003 American Institute of Physics.
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87.80.-y Biophysical techniques (research methods)
06.30.Dr Mass and density
85.85.+j Micro- and nano-electromechanical systems (MEMS/NEMS) and devices

Dynamics of liposomes gene vectors studied by anelastic spectroscopy

C. Castellano, D. Pozzi, G. Caracciolo, and R. Cantelli

Appl. Phys. Lett. 83, 2701 (2003); http://dx.doi.org/10.1063/1.1611651 (3 pages) | Cited 6 times

Online Publication Date: 23 September 2003

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The anelastic spectra of synthetic liposomes interesting for gene transfection have been measured in a wide temperature range; in particular, we have studied the cationic/neutral lipid mixture di-oleoyl trimethylammonium propane/di-oleoyl phosphatidylethanolamine at different molar ratios. This technique has been applied on thin films of biological samples deposited on a solid substrate like Si 〈100〉. We have evidenced the presence of two relaxation processes around and below 200 K, likely connected with a cooperative dynamics of the water plus membrane system. In particular, the process centered at 198 K results to be clearly a favorite by the presence of two different lipidic species. © 2003 American Institute of Physics.
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87.14.Cc Lipids
87.16.D- Membranes, bilayers, and vesicles
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