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18 Aug 2003

Volume 83, Issue 7, pp. 1283-1488

Issue Cover Spotlight Figure

Appl. Phys. Lett. 83, 1462 (2003); http://dx.doi.org/10.1063/1.1601693 (3 pages)

X. Cartoixà, D. Z.-Y. Ting, and Y.-C. Chang
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Electric-field-pulse-assisted covalent immobilization of DNA in the nanosecond time scale

F. Fixe, R. Cabeça, V. Chu, D. M. F. Prazeres, G. N. M. Ferreira, and J. P. Conde

Appl. Phys. Lett. 83, 1465 (2003); http://dx.doi.org/10.1063/1.1594839 (3 pages) | Cited 10 times

Online Publication Date: 12 August 2003

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A single square voltage pulse is used to achieve the selective covalent bonding of DNA probes to a functionalized thin-film surface (SiO2) on a plastic substrate. Significant covalent bonding occurs with only 100 ns of pulse duration, representing a decrease in immobilization time of more than ten orders of magnitude from the 2 h needed without electric field. A systematic study of the effect of the duration and rise/fall times of the voltage pulse on the immobilization of DNA probe molecules is presented. Successful hybridization of the DNA probes immobilized using this technique with a complementary target strand was achieved. © 2003 American Institute of Physics.
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87.15.K- Molecular interactions; membrane-protein interactions
87.14.G- Nucleic acids
87.50.C- Static and low-frequency electric and magnetic fields effects
87.15.B- Structure of biomolecules

DNA size separation using artificially nanostructured matrix

M. Baba, T. Sano, N. Iguchi, K. Iida, T. Sakamoto, and H. Kawaura

Appl. Phys. Lett. 83, 1468 (2003); http://dx.doi.org/10.1063/1.1602555 (3 pages) | Cited 24 times

Online Publication Date: 12 August 2003

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We have demonstrated two types of size separation of biomolecules using a nanostructured matrix artificially fabricated using electron-beam lithography: sieve-type separation using a regular pillar array structure and size exclusion chromatography (SEC) type separation using a structure with narrow and wide gaps. With these devices, samples of double-stranded DNA molecules (2, 5, and 10 k base pairs) were clearly separated into bands; smaller molecules eluted earlier in the sieve type while they eluted later in the SEC type. The nanostructured matrix enables various types of molecular separation by changing the design of the nanostructure. Moreover, it should be easy to integrate the matrix with other biomolecular fluidic devices because it does not require a filling medium. © 2003 American Institute of Physics.
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87.14.G- Nucleic acids
87.15.-v Biomolecules: structure and physical properties
87.85.Qr Nanotechnologies-design
87.85.Rs Nanotechnologies-applications
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