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27 Dec 2004

Volume 85, Issue 26, pp. 6323-6432

Issue Cover Spotlight Figure

Appl. Phys. Lett. 85, 6409 (2004); http://dx.doi.org/10.1063/1.1839274 (3 pages)

Peter D. D. Schwindt, Svenja Knappe, Vishal Shah, Leo Hollberg, John Kitching, Li-Anne Liew, and John Moreland
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Protein patterning by micromachined silicon embossing on polymer surfaces

D. Goustouridis, K. Misiakos, P. S. Petrou, and S. E. Kakabakos

Appl. Phys. Lett. 85, 6418 (2004); http://dx.doi.org/10.1063/1.1841458 (3 pages) | Cited 1 time

Online Publication Date: 17 December 2004

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An automated method for creating micron-sized patterns of different proteins is described. Unlike other methods, the process requires no masks, photochemichal reagents, or alignment. The basic tools are a motorized x–y stage, a pneumatic drive, and a micromachined silicon chip with an array of micron scale pyramids. Upon pressing the chip on a plastic slide, inverted pyramids are created on the polymer surface, exposing areas that have no previous coating and can adsorb proteins. The method is suitable for the fast and inexpensive fabrication of dense arrays of different proteins on appropriate substrates.
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87.14.E- Proteins
81.16.Rf Micro- and nanoscale pattern formation
87.15.M- Spectra of biomolecules
81.65.Cf Surface cleaning, etching, patterning
78.55.Kz Solid organic materials

Addressable micropost array for the dielectrophoretic manipulation of particles in fluid

T. P. Hunt, H. Lee, and R. M. Westervelt

Appl. Phys. Lett. 85, 6421 (2004); http://dx.doi.org/10.1063/1.1840109 (3 pages) | Cited 18 times

Online Publication Date: 17 December 2004

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A microfluidic device has been developed to trap and position neutral particles using dielectrophoresis. An array of microscale post shaped electrodes provides an inhomogeneous electric field. The voltage on each electrode can be independently controlled by a computer to trap and move particles in fluid above the micropost electrodes. Yeast cells and polystyrene spheres were trapped and moved, demonstrating both positive and negative dielectrophoresis.
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85.85.+j Micro- and nano-electromechanical systems (MEMS/NEMS) and devices
87.15.Tt Electrophoresis
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