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21 Mar 2005

Volume 86, Issue 12, Articles (12xxxx)

Issue Cover Spotlight Figure

Appl. Phys. Lett. 86, 123102 (2005); http://dx.doi.org/10.1063/1.1885187 (3 pages)

Jong H. Na, Robert A. Taylor, James H. Rice, James W. Robinson, Kwan H. Lee, Young S. Park, Chang M. Park, and Tae W. Kang
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Probing local water contents of in vitro protein films by ultrasonic force microscopy

Robert Szoszkiewicz, Andrzej J. Kulik, Gerard Gremaud, and Malgorzata Lekka

Appl. Phys. Lett. 86, 123901 (2005); http://dx.doi.org/10.1063/1.1891283 (3 pages) | Cited 7 times

Online Publication Date: 17 March 2005

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By means of ultrasonic force microscopy and lateral force microscopy we measure adhesion hysteresis and friction on protein films of bovine serum albumin and concanavalin A at local scales. Our investigations at different relative humidities (less than 5% and at 50% relative humidity) correspond to dehydrated and hydrated states of proteins. We demonstrate that a substantial increase of adhesion hysteresis with relative humidity is sensitive measure of protein–water binding capacity at local scales.
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87.80.-y Biophysical techniques (research methods)
87.15.La Mechanical properties
87.14.E- Proteins
87.64.Dz Scanning tunneling and atomic force microscopy
07.07.Vx Hygrometers; hygrometry
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