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4 Apr 2005

Volume 86, Issue 14, Articles (14xxxx)

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Appl. Phys. Lett. 86, 142101 (2005); http://dx.doi.org/10.1063/1.1895476 (3 pages)

M. Hanke, T. Boeck, A.-K. Gerlitzke, F. Syrowatka, F. Heyroth, and R. Köhler
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Field approach to three-dimensional gene expression pattern characterization

L. da F. Costa, B. A. N. Travençolo, A. Azeredo, M. E. Beletti, G. B. Müller, D. Rasskin-Gutman, G. Sternik, M. Ibañes, and J. C. Izpisúa-Belmonte

Appl. Phys. Lett. 86, 143901 (2005); http://dx.doi.org/10.1063/1.1898424 (3 pages) | Cited 3 times

Online Publication Date: 31 March 2005

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Show Abstract
We present a vector field method for obtaining the spatial organization of three-dimensional patterns of gene expression based on gradients and lines of force obtained by numerical integration. The convergence of these lines of force in local maxima are centers of gene expression, providing a natural and powerful framework to characterize the organization and dynamics of biological structures. We apply this methodology to analyze the expression pattern of the enhanced green fluorescent protein (EGFP) driven by the promoter of light chain myosin II during zebrafish heart formation.
Show PACS
87.14.E- Proteins
87.15.A- Theory, modeling, and computer simulation
87.19.Hh Cardiac dynamics
87.15.M- Spectra of biomolecules
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