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10 Jan 2005

Volume 86, Issue 2, Articles (02xxxx)

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Appl. Phys. Lett. 86, 021101 (2005); http://dx.doi.org/10.1063/1.1849439 (3 pages)

H. W. Choi, C. W. Jeon, C. Liu, I. M. Watson, M. D. Dawson, P. R. Edwards, R. W. Martin, S. Tripathy, and S. J. Chua
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Photoemission study of poly(dA)–poly(dT) DNA: Experimental and theoretical approach to the electronic density of states

Hiroki Wadati, Kozo Okazaki, Yasuhiro Niimi, Atsushi Fujimori, Hitoshi Tabata, Jared Pikus, and James P. Lewis

Appl. Phys. Lett. 86, 023901 (2005); http://dx.doi.org/10.1063/1.1850187 (3 pages) | Cited 7 times

Online Publication Date: 30 December 2004

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We present results of an ultraviolet photoemission spectroscopy study of artificially synthesized poly(dA)–poly(dT) DNA molecules on p-type Si substrates. For comparison, we also present the electronic density of states calculated using an ab initio tight-binding method based on density-functional theory (DFT). Good agreement was obtained between experiment and theory. The spectra of DNA networks on the Si substrate showed that the Fermi level of the substrate is located in the middle of the band gap of DNA. The spectra of thick ( ∼ 70 nm) DNA films showed a downward shift of ∼ 2 eV compared to the network samples.
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87.14.G- Nucleic acids
87.15.B- Structure of biomolecules
87.15.M- Spectra of biomolecules

Nonlinear dielectric spectroscopy of live cells using superconducting quantum interference devices

D. Nawarathna, J. R. Claycomb, J. H. Miller, and M. J. Benedik

Appl. Phys. Lett. 86, 023902 (2005); http://dx.doi.org/10.1063/1.1844036 (3 pages) | Cited 9 times

Online Publication Date: 3 January 2005

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Magnetic detection of the nonlinear response of cell suspensions to oscillating electrical fields is reported. It has been shown that H+-ATPase, which is located in plasma membrane of yeast cells, generates harmonics of the fundamental frequency when electrically excited at certain frequencies and field strengths. Electrode polarization may cause erroneous results, especially when using a conventional four-probe setup. In this letter we use highly sensitive superconducting quantum interference device magnetometers to detect the cells’ nonlinear response and to avoid electrode polarization effects. Experiments were carried out using yeast (Saccharomyces cerevisiae, 108 cells/ml) cells with excitation voltages and frequencies between 1–5V/cm and 10–300 Hz, respectively.
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87.16.D- Membranes, bilayers, and vesicles
82.70.Kj Emulsions and suspensions
85.25.Dq Superconducting quantum interference devices (SQUIDs)

Self-assembly of protein at aqueous solution surface in correlation to protein crystallization

Yanwei Jia and Xiang-Yang Liu

Appl. Phys. Lett. 86, 023903 (2005); http://dx.doi.org/10.1063/1.1846153 (3 pages) | Cited 6 times

Online Publication Date: 5 January 2005

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The assembly of lysozyme (hen egg white) at the surface of aqueous solution follows the same behaviors as amphiphilic molecules. The critical assembly concentration appearing in the protein solutions is found to coincide with the equilibrium concentration of protein crystals under given conditions. The crystallization of protein regarded as a typical case of protein self-assembly in three dimensions has been discussed. The result reveals also the correlation between protein crystallization and the two-dimensional self-assembly at the surface of substrates. It follows that the protein crystallization condition can be determined without protein crystals.
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87.14.E- Proteins
87.15.N- Properties of solutions of macromolecules
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