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24 Jan 2005

Volume 86, Issue 4, Articles (04xxxx)

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Appl. Phys. Lett. 86, 043106 (2005); http://dx.doi.org/10.1063/1.1853514 (3 pages)

William L. Hughes and Zhong L. Wang
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Mechanical perturbation-induced fluorescence change of green fluorescent protein

Takashi Kodama, Hiroyuki Ohtani, Hideo Arakawa, and Atsushi Ikai

Appl. Phys. Lett. 86, 043901 (2005); http://dx.doi.org/10.1063/1.1856142 (3 pages) | Cited 13 times

Online Publication Date: 20 January 2005

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The force curve measurement mode of the atomic force microscope (AFM) is a powerful experimental technique in biotechnology. However, it is more effective if the spectroscopic properties of the biomolecule in the contact area can be simultaneously measured. Thus, we developed a confocal laser scanning microscope/AFM system. In this study, we simultaneously measured the fluorescence spectra of green fluorescent protein with the application of an external force in order to investigate the stability and dynamics of the β-barrel structure. Consequently, the fluorescence was quenched by applying both compression and extension forces and the quenching efficiencies differed in each case.
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87.80.-y Biophysical techniques (research methods)
07.79.Lh Atomic force microscopes
87.14.E- Proteins
87.64.Dz Scanning tunneling and atomic force microscopy
87.15.M- Spectra of biomolecules
87.15.H- Dynamics of biomolecules
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