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12 Dec 2005

Volume 87, Issue 24, Articles (24xxxx)

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Appl. Phys. Lett. 87, 243101 (2005); http://dx.doi.org/10.1063/1.2147713 (3 pages)

Y.-S. Choi, K. Hennessy, R. Sharma, E. Haberer, Y. Gao, S. P. DenBaars, S. Nakamura, E. L. Hu, and C. Meier
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Single-pulse cell stimulation with a near-infrared picosecond laser

Shigeki Iwanaga, Nicholas Smith, Katsumasa Fujita, Satoshi Kawata, and Osamu Nakamura

Appl. Phys. Lett. 87, 243901 (2005); http://dx.doi.org/10.1063/1.2147733 (3 pages) | Cited 1 time

Online Publication Date: 6 December 2005

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We have demonstrated stimulation of living cells by picosecond laser pulses. HeLa cells were exposed to focused 1.7 ps pulses in a pulse train of between 1 and 16 pulses with different repetition rates. The pulses were generated by a titanium–sapphire laser and regenerative amplifier with a wavelength of 775 nm. Contrary to expectation, we found that for a short pulse train (between 1 and 16 pulses at differing repetition rates), only the first pulse is responsible for triggering intracellular Ca2+ waves. These results show that the technique can be used to stimulate a calcium response in a living cell without thermal energy depositions.
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87.50.W- Optical/infrared radiation effects
87.16.D- Membranes, bilayers, and vesicles
87.16.Uv Active transport processes

Microfabricated high-performance microwave impedance biosensors for detection of aptamer-protein interactions

M. Löhndorf, U. Schlecht, T. M. A. Gronewold, A. Malavé, and M. Tewes

Appl. Phys. Lett. 87, 243902 (2005); http://dx.doi.org/10.1063/1.2146058 (3 pages) | Cited 12 times

Online Publication Date: 8 December 2005

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High-frequency impedance biosensors with nanometer gaps have been prepared for the detection of biomolecular interactions such as protein-antibody and protein-aptamer binding. The sensor principle is based on electrical impedance changes measured at 1.2 GHz due to changes of the effective dielectric constant within the 68 nm gaps between two gold electrodes. As a model system, the specific binding of the blood clotting factor human thrombin with different concentrations to its ribonucleic acid (RNA) α-thrombin aptamer, as well as the immobilization process of the RNA-aptamer, have been detected in real time. By using a similar 68 nm-gap sensor blocked with bovine serum albumin and a reference sensor with 10 μm electrode spacing, signal changes due to variations of the bulk dielectric constant due to buffer/analyte solutions, and unspecific binding events have been analyzed.
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87.80.-y Biophysical techniques (research methods)
87.15.K- Molecular interactions; membrane-protein interactions
87.14.E- Proteins
07.07.Df Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing
07.10.Cm Micromechanical devices and systems
07.57.Kp Bolometers; infrared, submillimeter wave, microwave, and radiowave receivers and detectors
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