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26 Dec 2005

Volume 87, Issue 26, Articles (26xxxx)

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Appl. Phys. Lett. 87, 263102 (2005); http://dx.doi.org/10.1063/1.2150278 (3 pages)

Z. Zhong, G. Katsaros, M. Stoffel, G. Costantini, K. Kern, O. G. Schmidt, N. Y. Jin-Phillipp, and G. Bauer
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Chemical functionalization of GaN and AlN surfaces

B. Baur, G. Steinhoff, J. Hernando, O. Purrucker, M. Tanaka, B. Nickel, M. Stutzmann, and M. Eickhoff

Appl. Phys. Lett. 87, 263901 (2005); http://dx.doi.org/10.1063/1.2150280 (3 pages) | Cited 22 times

Online Publication Date: 22 December 2005

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The covalent functionalization of GaN and AlN surfaces with organosilanes is demonstrated. Both octadecyltrimethoxysilane and aminopropyltriethoxysilane form self-assembled monolayers on hydroxylated GaN and AlN surfaces, confirmed by x-ray photoelectron spectroscopy and atomic force microscopy. The monolayer thickness on GaN was determined to 2.5±0.2 nm by x-ray reflectivity. Temperature-programmed desorption measurements reveal a desorption enthalpy of 240 kJ/mol. The realization of micropatterned self-assembled monolayers and the hybridization of deoxyribonucleic acid molecules on biofunctionalized GaN surfaces are shown.
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87.80.-y Biophysical techniques (research methods)
87.14.G- Nucleic acids
87.15.-v Biomolecules: structure and physical properties
68.47.Pe Langmuir-Blodgett films on solids; polymers on surfaces; biological molecules on surfaces
82.80.Pv Electron spectroscopy (X-ray photoelectron (XPS), Auger electron spectroscopy (AES), etc.)
68.43.Vx Thermal desorption

Nanofilter array chip for fast gel-free biomolecule separation

Jianping Fu, Pan Mao, and Jongyoon Han

Appl. Phys. Lett. 87, 263902 (2005); http://dx.doi.org/10.1063/1.2149979 (3 pages) | Cited 50 times

Online Publication Date: 22 December 2005

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We report here a microfabricated nanofilter array chip that can size-fractionate sodium dodecyl sulfate (SDS)-protein complexes and small DNA molecules based on the Ogston sieving mechanism. Nanofilter arrays with a gap size of 40–180 nm were fabricated and characterized. Complete separation of SDS-protein complexes and small DNA molecules were achieved in several minutes with a separation length of 5 mm. The fabrication strategy for the nanofilter array chip allows further increasing of the nanofilter density and decreasing of the nanofilter gap size, leading, in principle, to even faster separation.
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87.80.-y Biophysical techniques (research methods)
87.14.E- Proteins
87.14.G- Nucleic acids
36.20.-r Macromolecules and polymer molecules

Thinning out clusters while conserving stoichiometry of labeling

Manuel Moertelmaier, Mario Brameshuber, Mario Linimeier, Gerhard J. Schütz, and Hannes Stockinger

Appl. Phys. Lett. 87, 263903 (2005); http://dx.doi.org/10.1063/1.2158031 (3 pages) | Cited 13 times

Online Publication Date: 23 December 2005

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Here we present a method for the stoichiometric analysis of molecular aggregates in the cellular plasma membrane, based on single molecule fluorescence microscopy. We use selective photobleaching to erase all active fluorophores within a small region of the membrane, while conserving the stoichiometry of labeling in the remaining part of the membrane. At the onset of repopulation due to Brownian motion, single diffraction limited spots of individual aggregates can be resolved and quantified. We demonstrate the proof of principle of this method by quantifying the dye load of fluorescently labeled immunoglobulins diffusing in a supported lipid bilayer.
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87.15.B- Structure of biomolecules
87.16.D- Membranes, bilayers, and vesicles
87.14.Cc Lipids
33.50.Dq Fluorescence and phosphorescence spectra
87.15.M- Spectra of biomolecules
05.40.Jc Brownian motion
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