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11 Jul 2005

Volume 87, Issue 2, Articles (02xxxx)

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Appl. Phys. Lett. 87, 024103 (2005); http://dx.doi.org/10.1063/1.1984098 (3 pages)

A. Dupuis, J. Léopoldès, D. G. Bucknall, and J. M. Yeomans
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Three-dimensional confocal thermal imaging using anti-Stokes luminescence

A. V. Kachynski, A. N. Kuzmin, H. E. Pudavar, and P. N. Prasad

Appl. Phys. Lett. 87, 023901 (2005); http://dx.doi.org/10.1063/1.1993761 (3 pages) | Cited 5 times

Online Publication Date: 6 July 2005

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High-resolution thermal images of dye-stained live cells are obtained using confocal anti-Stokes luminescence on a confocal laser scanning microscope. Stokes and anti-Stokes fluorescence signal ratio imaging, using dual laser excitation with a fast alternate line scan, is used to monitor dynamical temperature changes at submicrometer resolution. Temperature mapping of a dye-doped polymer film with local heating is shown as an example. Three-dimensional mapping of local temperature changes in living cells induced by an external laser as the heating source, is obtained with temperature resolution better than 0.5 K.
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87.80.-y Biophysical techniques (research methods)
87.17.-d Cell processes
87.64.mk Confocal
87.19.Pp Biothermics and thermal processes in biology
87.15.M- Spectra of biomolecules
87.50.W- Optical/infrared radiation effects
33.50.Dq Fluorescence and phosphorescence spectra
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