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6 Mar 2006

Volume 88, Issue 10, Articles (10xxxx)

Issue Cover Spotlight Figure

Appl. Phys. Lett. 88, 103107 (2006); http://dx.doi.org/10.1063/1.2182096 (3 pages)

A. B. Djurišić, Y. H. Leung, K. H. Tam, L. Ding, W. K. Ge, H. Y. Chen, and S. Gwo
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Probing messenger RNA conformational heterogeneity using single-molecule fluorescence anisotropy

Deepak Sinha, Srikanth Sastry, and G. V. Shivashankar

Appl. Phys. Lett. 88, 103901 (2006); http://dx.doi.org/10.1063/1.2183358 (3 pages) | Cited 1 time

Online Publication Date: 7 March 2006

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In this letter we describe a method to probe biomolecular conformations and their dynamics at the single molecule level. We show, using fluorescence anisotropy based methods, that the hydrodynamic volume of biomolecules captures the intrinsic heterogeneity within a population. Population distributions of conformations and their dynamics are studied by making anisotropy measurements on one molecule at a time within a confocal volume. The mean anisotropy of mRNA is lowered on addition of salt while the spread remains the same. The intrinsic heterogeneity is revealed when conformational transitions are frozen, resulting in a drastic increase in the spread of the anisotropy. These studies reveal that mRNA samples a broad range of conformations.
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87.64.K- Spectroscopy
87.14.G- Nucleic acids
87.15.H- Dynamics of biomolecules
87.15.M- Spectra of biomolecules
33.50.Dq Fluorescence and phosphorescence spectra

Ultrahigh-speed optical frequency domain imaging and application to laser ablation monitoring

W. Y. Oh, S. H. Yun, B. J. Vakoc, G. J. Tearney, and B. E. Bouma

Appl. Phys. Lett. 88, 103902 (2006); http://dx.doi.org/10.1063/1.2179125 (3 pages) | Cited 22 times

Online Publication Date: 10 March 2006

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We demonstrate a linear laser resonator incorporating a semiconductor optical amplifier and scanning filter for high repetition rate, broad wavelength, unidirectional scanning. The laser operates at up to 115 kHz repetition rates and demonstrates a tuning-speed-independent power of >30 mW. We apply this laser to enable ultrahigh-speed optical frequency domain imaging of the dynamics of laser ablation of biological tissue. The imaging system acquires single longitudinal scans (A-lines) in 8.7 μs and complete cross-sectional images comprising 575 A-lines at a rate of 200 frames per second.
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87.63.L- Visual imaging
42.62.Be Biological and medical applications
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