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6 Feb 2006

Volume 88, Issue 6, Articles (06xxxx)

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Appl. Phys. Lett. 88, 063509 (2006); http://dx.doi.org/10.1063/1.2171834 (3 pages)

M. Feng, N. Holonyak, R. Chan, A. James, and G. Walter
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Spectral signature of native CN bonds for bacterium detection and identification using femtosecond laser-induced breakdown spectroscopy

Matthieu Baudelet, Laurent Guyon, Jin Yu, Jean-Pierre Wolf, Tanguy Amodeo, Emeric Fréjafon, and Patrick Laloi

Appl. Phys. Lett. 88, 063901 (2006); http://dx.doi.org/10.1063/1.2170437 (3 pages) | Cited 36 times

Online Publication Date: 7 February 2006

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A sample of Escherichia coli has been analyzed by laser-induced breakdown spectroscopy (LIBS) using femtosecond pulses. The spectrum shows strong CN molecular bands due to the direct ablation of native CN molecular bonds from the bacteria in contrast with weak atomic lines from carbon. The native nature of the observed CN bonds is supported by the kinetic behavior of the CN band head which rapidly decays with a time constant of 94 ns, while for a pure graphite sample the CN band head increases with a delay of 450 ns due to recombination with the ambient air. Moreover, about hundred resolved lines belonging to 12 atomic or molecular species are recorded, providing a valuable spectral signature to identify the bacterium.
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87.64.-t Spectroscopic and microscopic techniques in biophysics and medical physics
82.53.Ps Femtosecond probing of biological molecules
33.15.Fm Bond strengths, dissociation energies

Anomalous temperature dependence of electrical conductance of DNA-linked Au nanoparticle aggregates

Yong Ju Yun, Gwangseo Park, Sunkyung Jung, and Dong Han Ha

Appl. Phys. Lett. 88, 063902 (2006); http://dx.doi.org/10.1063/1.2172021 (3 pages) | Cited 5 times

Online Publication Date: 9 February 2006

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We have measured the electrical conductance of DNA-linked Au nanoparticle aggregates using the four probe method. FE-SEM images show that the Au nanoparticles are remained intact but not fused together. As the temperature increases under a constant relative humidity, the electrical conductance of Au nanoparticle aggregates linked by 12 base DNA molecules increases exponentially with an anomaly around the melting temperature of the linker DNAs due to the abrupt change of the amount of water molecules adsorbed on DNAs, but we could not observe such an anomaly for the aggregates linked by 24 base DNAs up to 71 °C. We expect our results can be utilized as a DNA detection method which does not require any thermal-stringency wash before the measurement at each temperature.
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87.80.-y Biophysical techniques (research methods)
87.14.G- Nucleic acids
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