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Applied Physics Letters / Volume 89 / Issue 13 / APPLIED BIOPHYSICS

Appl. Phys. Lett. 89, 133901 (2006); http://dx.doi.org/10.1063/1.2357885 (3 pages)

Stretching of long DNA molecules in the microvortex induced by laser and ac electric field

Michihiko Nakano1, Hirofumi Kurita1, Jun Komatsu1, Akira Mizuno1, and Shinji Katsura2

1Department of Ecological Engineering, Toyohashi University of Technology, Aichi 441-8580, Japan
2Department of Biological and Chemical Engineering, Gunma University, Gunma 376-0052, Japan

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(Received 10 March 2006; accepted 16 August 2006; published online 26 September 2006)

A microvortex is generated around an infrared laser focus where an intense ac electric field is applied. The authors used this optoelectrostatic microvortex for stretching individual long DNAs. When λ-or T4-phage DNA molecules were introduced into the optoelectrostatic microvortex, they were stretched around the laser focus. In addition, especially for longer T4 DNA molecules, it was possible to keep it in stretching form for more than 30 s. Using this method, length of DNA molecules can be measured without fixing to a substrate. This method can be applied to DNA molecules longer than about 10 μm.

© 2006 American Institute of Physics

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KEYWORDS and PACS

Keywords

DNA, molecular biophysics, biological techniques, bioelectric phenomena, vortices, biological effects of laser radiation, biological effects of fields

PACS

  • 87.50.W-

    Optical/infrared radiation effects

  • 87.50.C-

    Static and low-frequency electric and magnetic fields effects

  • 87.14.G-

    Nucleic acids

  • 47.32.-y

    Vortex dynamics; rotating fluids

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.2357885

PUBLICATION DATA

ISSN

0003-6951 (print)  
1077-3118 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    Y.-J. Juang, S. Wang, X. Hu, and L. J. Lee, Phys. Rev. Lett. 93, 268105 (2004).

    N. Kaji, M. Ueda, and Y. Baba, Appl. Phys. Lett. 83, 3413 (2003)APPLAB000083000016003413000001.


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