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7 Aug 2006

Volume 89, Issue 6, Articles (06xxxx)

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Appl. Phys. Lett. 89, 062501 (2006); http://dx.doi.org/10.1063/1.2259813 (3 pages)

Sangkook Choi, Ki-Suk Lee, and Sang-Koog Kim
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Excitation of fluorescence decay using a 265 nm pulsed light-emitting diode: Evidence for aqueous phenylalanine rotamers

Colin D. McGuinness, Alexander M. Macmillan, Kulwinder Sagoo, David McLoskey, and David J. S. Birch

Appl. Phys. Lett. 89, 063901 (2006); http://dx.doi.org/10.1063/1.2245441 (3 pages) | Cited 11 times

Online Publication Date: 10 August 2006

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The authors describe the characteristics and application of a 265 nm AlGaN light-emitting diode (LED) operated at 1 MHz repetition rate, 1.2 ns pulse duration, 1.32 μW average power, 2.3 mW peak power, and ∼ 12 nm bandwidth. The LED enables the fluorescence decay of weakly emitting phenylalanine to be measured routinely, even in dilute solution. For pH of 6–9.2, the authors find evidence for a biexponential rather than monoexponential decay, providing direct evidence for the presence of phenylalanine rotamers with a photophysics closer to the other two fluorescent amino acids tryrosine and tryptophan than has previously been reported.
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87.80.-y Biophysical techniques (research methods)
85.60.Jb Light-emitting devices
87.15.M- Spectra of biomolecules
33.50.Dq Fluorescence and phosphorescence spectra
33.80.-b Photon interactions with molecules
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