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1 Jan 2007

Volume 90, Issue 1, Articles (01xxxx)

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Appl. Phys. Lett. 90, 012105 (2007); http://dx.doi.org/10.1063/1.2428402 (3 pages)

Jan Bauer, Frank Fleischer, Otwin Breitenstein, Luise Schubert, Peter Werner, Ulrich Gösele, and Margit Zacharias
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In vivo real-time ethanol vapor detection in the interstitial fluid of a Wistar rat using piezoresistive microcantilevers

C. Parks Cheney, A. Wig, R. H. Farahi, A. Gehl, D. L. Hedden, T. L. Ferrell, D. Ji, R. Bell, W. J. McBride, and S. O’Connor

Appl. Phys. Lett. 90, 013901 (2007); http://dx.doi.org/10.1063/1.2400104 (3 pages) | Cited 4 times

Online Publication Date: 2 January 2007

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Real-time, in vivo measurements were taken in the interstitial fluid of a Wistar rat after administering 2.5 g/kg ethanol by intraperitoneal injection. A low-power piezoresistive microcantilever sensor array was used with polymer coatings suitable for measuring ethanol concentrations at 100% humidity over several hours. A hydrophobic, vapor permeable nanopore membrane was used to screen liquid and ions while allowing vapor to pass to the sensor that was implanted into the saline environment presented by the interstitial fluid. The real-time measurements followed the time scale of previous blood ethanol concentration data.
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87.80.-y Biophysical techniques (research methods)
85.85.+j Micro- and nano-electromechanical systems (MEMS/NEMS) and devices
82.80.-d Chemical analysis and related physical methods of analysis
07.07.Df Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing
07.10.Cm Micromechanical devices and systems

Dynamic optimization of on-chip polymerase chain reaction by monitoring intracycle fluorescence using fast synchronous detection

Sudip Mondal, Debjani Paul, and V. Venkataraman

Appl. Phys. Lett. 90, 013902 (2007); http://dx.doi.org/10.1063/1.2430628 (3 pages) | Cited 1 time

Online Publication Date: 4 January 2007

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The authors report on-chip dynamic optimization of polymerase chain reaction (PCR) based on a feedback technique utilizing synchronous detection of intracycle fluorescence every 500 ms. From a direct measurement of polymerase activity, the authors determine the optimum extension temperature. The authors dynamically optimize PCR in an inductively heated microchip by sensing the saturation of extension in each cycle and applying the feedback. They demonstrate that, even with fast ramp rates, dynamic optimization leads to faster reactions compared to fixed-duration extension protocols for long DNA (>500 bp). This optimization scheme uses a fairly universal dye Sybr Green I and can be applied to most PCRs.
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87.15.R- Reactions and kinetics
87.14.G- Nucleic acids
33.50.Dq Fluorescence and phosphorescence spectra
87.64.K- Spectroscopy
87.80.-y Biophysical techniques (research methods)

Protein destruction by atmospheric pressure glow discharges

X. T. Deng, J. J. Shi, H. L. Chen, and M. G. Kong

Appl. Phys. Lett. 90, 013903 (2007); http://dx.doi.org/10.1063/1.2410219 (3 pages) | Cited 59 times

Online Publication Date: 4 January 2007

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It is well established that atmospheric pressure glow discharges are capable of bacterial inactivation. Much less known is their ability to destruct infectious proteins, even though surgical instruments are often contaminated by both bacteria and proteinaceous matters. In this letter, the authors present a study of protein destruction using a low-temperature atmospheric dielectric-barrier discharge jet. Clear evidences of protein removal are presented with data of several complimentary experiments using scanning electron microscopy, electron dispersive x-ray analysis, electrophoresis, laser-induced fluorescence microscopy, and protein reduction kinetics. Considerable degradation is observed of protein fragments that remain on their substrate surface after plasma treatment.
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87.14.E- Proteins
82.80.Ej X-ray, Mössbauer, and other γ-ray spectroscopic analysis methods
87.15.Tt Electrophoresis
87.50.-a Effects of electromagnetic and acoustic fields on biological systems
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