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29 Jan 2007

Volume 90, Issue 5, Articles (05xxxx)

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Appl. Phys. Lett. 90, 052503 (2007); http://dx.doi.org/10.1063/1.2436715 (3 pages)

Biqin Huang, Igor Altfeder, and Ian Appelbaum
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Imaging of hemoglobin oxygen saturation variations in single vessels in vivo using photoacoustic microscopy

Hao F. Zhang, Konstantin Maslov, Mathangi Sivaramakrishnan, Gheorghe Stoica, and Lihong V. Wang

Appl. Phys. Lett. 90, 053901 (2007); http://dx.doi.org/10.1063/1.2435697 (3 pages) | Cited 80 times

Online Publication Date: 29 January 2007

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Photoacoustic microscopy was used to noninvasively image variations in hemoglobin oxygen saturation (SO2) in the subcutaneous microvasculature of rats in vivo. In phantom tests, the calculated concentration fractions of red ink in double-ink mixtures matched the actual values with a 1% error. In ex vivo studies, the calculated SO2 in bovine blood agreed with the standard spectrophotometric measurements within a 4% systematic difference. In in vivo studies, arteries and veins were separated based on the measured SO2 values and variations in SO2 between different physiological states (hyperoxia, normoxia, and hypoxia) were imaged in single blood vessels.
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87.63.L- Visual imaging
87.63.D- Ultrasonography
87.14.E- Proteins
43.80.Qf Medical diagnosis with acoustics

Nanometric three-dimensional tracking of individual quantum dots in cells

Laurent Holtzer, Tobias Meckel, and Thomas Schmidt

Appl. Phys. Lett. 90, 053902 (2007); http://dx.doi.org/10.1063/1.2437066 (3 pages) | Cited 51 times

Online Publication Date: 1 February 2007

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Wide-field single-molecule fluorescence microscopy has become an established tool for the study of dynamic biological processes which occur in the plane of a cellular membrane. In the current study we have extended this technique to the three-dimensional analysis of molecular mobility. Introduction of a cylindrical lens into the emission path of a microscope produced some astigmatism which was used to obtain the full three-dimensional position information. The localization accuracy of fluorescent objects was calculated theoretically and subsequently confirmed by simulations and by experiments. For further validation individual quantum dots were followed when passively diffusing and actively transported within life cells.
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87.64.K- Spectroscopy
87.80.-y Biophysical techniques (research methods)
87.15.Vv Diffusion
87.16.D- Membranes, bilayers, and vesicles
33.50.Dq Fluorescence and phosphorescence spectra

Optical and electrical properties of [N,N′-bis(salicylidene)-ethylenediamine]zinc as an electroluminescent material

Bingshe Xu, Yuying Hao, Xiaohong Fang, Hua Wang, and Xuguang Liu

Appl. Phys. Lett. 90, 053903 (2007); http://dx.doi.org/10.1063/1.2450657 (3 pages) | Cited 6 times

Online Publication Date: 1 February 2007

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The variability of photoluminescence (PL) and electroluminescence (EL) properties of [N,N′-bis(salicylidene)-ethylenediamine]zinc [Zn(salen)] was investigated. It was found that the PL spectra of Zn(salen) powder are dependent on synthesis temperature, and recrystallization solvent and those of Zn(salen) film are dependent on deposition vacuum degree. The EL properties of Zn(salen) are not only dependent on deposition vacuum degree but also dependent on driving voltage. The variability of PL and EL properties of Zn(salen) can be related to the various molecular geometries of Zn(salen).
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78.66.Qn Polymers; organic compounds
78.60.Fi Electroluminescence
78.55.Kz Solid organic materials

Interphoton burst recurrence times: Single cell analysis in freely flowing solutions

Joshua B. Edel and Andrew J. deMello

Appl. Phys. Lett. 90, 053904 (2007); http://dx.doi.org/10.1063/1.2435327 (3 pages) | Cited 3 times

Online Publication Date: 2 February 2007

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The authors present a simple and direct analysis method for the discrimination between different cell populations in fluidic media. The methodology is based on analysis of single particle interphoton burst recurrence times and has potential use in high precision single cell sizing and counting applications. The approach requires registration of only a few hundred photons from single fluorescent particles to distinguish between different molecular populations. The technique is simple to implement and can be designed to extract information in real time within microfluidic environments.
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87.17.-d Cell processes
47.63.-b Biological fluid dynamics
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