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Applied Physics Letters / Volume 91 / Issue 11 / APPLIED BIOPHYSICS

Appl. Phys. Lett. 91, 113902 (2007); http://dx.doi.org/10.1063/1.2784967 (3 pages)

Separation of long DNA molecules through cleavage of hydrogen bonds under a stretching force

Lizeng Gao1, Jiamin Wu1, Di Gao1, and Jianzhong Wu2

1Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
2Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, USA

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(Received 26 July 2007; accepted 24 August 2007; published online 10 September 2007)

The authors report that long DNA molecules of different lengths can be separated under a stretching force by cleaving hydrogen bonds that tether one end of the DNA to a substrate. This separation method can be implemented with a simple direct current electric field, does not require separation matrices, and, in principle, has no upper limit on the length of the DNA that can be efficiently separated. Here, they demonstrate efficient separation of lambda DNA (48 502 base pairs) from human genomic DNA (>100 000 base pairs) using this method.

© 2007 American Institute of Physics

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KEYWORDS and PACS

Keywords

DNA, hydrogen bonds, molecular biophysics

PACS

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.2784967

PUBLICATION DATA

ISSN

0003-6951 (print)  
1077-3118 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    E. A. Di Marzio and C. M. Guttman, J. Chem. Phys. 95, 1189 (1991)JCPSA6000095000002001189000001.

    D. K. Lubensky and D. R. Nelson, Phys. Rev. E 65, 031917 (2002).


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