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24 Sep 2007

Volume 91, Issue 13, Articles (13xxxx)

Issue Cover Spotlight Figure

Appl. Phys. Lett. 91, 132501 (2007); http://dx.doi.org/10.1063/1.2786856 (3 pages)

V. Rose, X. M. Cheng, D. J. Keavney, J. W. Freeland, K. S. Buchanan, B. Ilic, and V. Metlushko
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Melting curve analysis in a snapshot

Philipp Baaske, Stefan Duhr, and Dieter Braun

Appl. Phys. Lett. 91, 133901 (2007); http://dx.doi.org/10.1063/1.2790806 (3 pages) | Cited 9 times

Online Publication Date: 26 September 2007

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The thermal denaturation of molecules is an essential method in biochemistry and diagnostics, including the measurement of single nucleotide polymorphisms and the binding analysis of proteins. We present a method for the all-optical high speed measurement of melting curves. A thin sheet of water is locally heated with an infrared laser to obtain a spatial temperature distribution between 20 and 100 °C. Using a fluorescence microscope a melting curve is recorded within 50 ms. This is about 10 000-times faster than state-of-the-art fluorometry and yields the same results for the validation example of a DNA hairpin.
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87.15.N- Properties of solutions of macromolecules
87.14.E- Proteins
87.14.G- Nucleic acids
87.15.H- Dynamics of biomolecules

Blue-light (488 nm)-irradiation-induced photoactivation of the photoactivatable green fluorescent protein

Ilaria Testa, Davide Mazza, Sara Barozzi, Mario Faretta, and Alberto Diaspro

Appl. Phys. Lett. 91, 133902 (2007); http://dx.doi.org/10.1063/1.2790847 (3 pages) | Cited 4 times

Online Publication Date: 26 September 2007

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We experimentally demonstrate the photoactivatable green fluorescent protein (paGFP) photoactivation in a wavelength range where the molecule barely absorbs. The photoactivation is induced at the same wavelength used to visualize the activated form of paGFP. This can be an obstacle in the intensity evaluation in photoactivation experiments. Power and kinetics based characterization of the effect was performed in model and cell systems. This study shows an operative threshold in which paGFP is not subjected to significant photoconversion. 488 nm photoactivation is in tune with the broadening of the paGFP two-photon activation spectrum, indicating that multiple interactions lead to modifications of the molecular structure and alterations of its photophysical properties.
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87.14.E- Proteins
87.15.M- Spectra of biomolecules
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