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1 Oct 2007

Volume 91, Issue 14, Articles (14xxxx)

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Appl. Phys. Lett. 91, 143101 (2007); http://dx.doi.org/10.1063/1.2789812 (3 pages)

Hyun S. Kim, Hua Qin, and Robert H. Blick
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Protein detection using a radio frequency biosensor with amplified gold nanoparticles

J. H. Chien, P. H. Chen, L. S. Kuo, C. S. Lin, and H. Wang

Appl. Phys. Lett. 91, 143901 (2007); http://dx.doi.org/10.1063/1.2794758 (3 pages) | Cited 2 times

Online Publication Date: 2 October 2007

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This study presents a device for protein detection using a low-pass radio frequency filter with gold nanoparticles (AuNPs). Self-assembled multilayer gold nanoparticles were immobilized on the sensing surface of the filter by sandwich immunoassay. The measured frequency of the filter ranges from 50 MHz to 30 GHz. The multilayer gold nanoparticles result in a change of 3 dB bandwidth of the low-pass filter. Results show that 1 ng/μl of RIgG solution can be detected after triple-layer AuNPs are completely formed. The change in 3 dB bandwidth increases with the concentration of target protein. Therefore, this device has potential for protein quantification.
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87.80.-y Biophysical techniques (research methods)
84.30.Vn Filters
87.85.Qr Nanotechnologies-design
87.85.Rs Nanotechnologies-applications
87.14.E- Proteins
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Magnetoimpedance biosensor for Fe3O4 nanoparticle intracellular uptake evaluation

A. Kumar, S. Mohapatra, V. Fal-Miyar, A. Cerdeira, J. A. García, H. Srikanth, J. Gass, and G. V. Kurlyandskaya

Appl. Phys. Lett. 91, 143902 (2007); http://dx.doi.org/10.1063/1.2790370 (3 pages) | Cited 13 times

Online Publication Date: 3 October 2007

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Iron oxide (Fe3O4) nonspecific nanoparticles of 30 nm are embedded inside human embryonic kidney (HEK 293) cells by intracellular uptake with a concentration of ∼ 105 particles/cell. An amorphous ribbon of Co64.5Fe2.5Cr3Si15B15 exhibiting large magnetoimpedance (MI) serves as the sensing element. The presence of fringing fields of the nanoparticles changes the superposition of the constant applied field and the alternating field created by a current flowing through the ribbon that can be detected as a change in MI. This response is clearly dependent on the presence of the magnetic nanoparticles inside the cells and on the value of the external field.
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87.80.-y Biophysical techniques (research methods)
87.85.Qr Nanotechnologies-design
87.85.Rs Nanotechnologies-applications
87.17.-d Cell processes
85.70.Kh Magnetic thin film devices: magnetic heads (magnetoresistive, inductive, etc.); domain-motion devices, etc.

On the correlation between phase evolution of lipoplexes/anionic lipid mixtures and DNA release

Giulio Caracciolo, Daniela Pozzi, Ruggero Caminiti, Cristina Marchini, Maura Montani, Augusto Amici, and Heinz Amenitsch

Appl. Phys. Lett. 91, 143903 (2007); http://dx.doi.org/10.1063/1.2794436 (3 pages) | Cited 1 time

Online Publication Date: 4 October 2007

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We investigated the structural evolution of three lipoplex formulations when interacting with anionic lipids by synchrotron small angle x-ray diffraction, while the extent of DNA release from lipoplexes by anionic lipids was evaluated by gel electrophoresis. Lipoplexes formed lamellar phases when mixed with anionic dioleoylphosphatidylglycerol (DOPG), while promoting the formation of nonbilayer structures when mixed with anionic dioleoylphosphatidic acid (DOPA). However, lipoplexes exhibited a virtually identical extent of DNA release when mixed with DOPG or DOPA. Thus, the recently proposed correlation between the formation of nonlamellar phases in lipoplex/anionic lipid mixtures and the increase of DNA release does not seem to exist.
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87.14.G- Nucleic acids
87.15.B- Structure of biomolecules
87.15.K- Molecular interactions; membrane-protein interactions
87.15.Tt Electrophoresis
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