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12 Nov 2007

Volume 91, Issue 20, Articles (20xxxx)

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Appl. Phys. Lett. 91, 203501 (2007); http://dx.doi.org/10.1063/1.2806922 (3 pages)

Michael N. Feiginov and Dibakar Roy Chowdhury
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Direct visualization of triplex DNA molecular dynamics by fluorescence resonance energy transfer and atomic force microscopy measurements

Chia-Ching Chang, Po-Yen Lin, Yen-Fu Chen, Chia-Seng Chang, and Lou-Sing Kan

Appl. Phys. Lett. 91, 203901 (2007); http://dx.doi.org/10.1063/1.2809406 (3 pages) | Cited 4 times

Online Publication Date: 13 November 2007

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We have detected the dynamics of 17-mer DNA triplex dissociation mechanism at the molecular level. Fluorescence resonance energy transfer (FRET) was used as an indicator of intermolecular interaction in nanometer range, whereas atomic force microscopy (AFM) was employed to address single molecule with sub-angstrom precision. The maximum rupture force of DNA triplex was found at pH 4.65, consistent with macroscopic observations. These results indicated that the FRET together with an AFM detection system could be used to reveal the DNA triplex interaction in nanometer scale unambiguously.
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61.43.Bn Structural modeling: serial-addition models, computer simulation

Real-time monitoring of cell death by surface infrared spectroscopy

Ryo-taro Yamaguchi, Ayumi Hirano-Iwata, Yasuo Kimura, Michio Niwano, Ko-ichiro Miyamoto, Hiroko Isoda, and Hitoshi Miyazaki

Appl. Phys. Lett. 91, 203902 (2007); http://dx.doi.org/10.1063/1.2813013 (3 pages) | Cited 6 times

Online Publication Date: 13 November 2007

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We have developed a method for real-time monitoring of the cell responses to cytotoxicants using Fourier transform infrared spectroscopy with the multiple internal reflection (MIR-FTIR) geometry. To prevent cell damages induced by measurement environments, we have constructed specialized chambers, in which temperature was maintained at (37±0.5) °C and humidified air containing 5% CO2 was supplied. We monitored cell death induced by cytotoxic surfactant Tween20 using MIR-FTIR spectroscopy. It was found that cell death can be monitored by the absorption intensity of amide II band. This result suggests that our method has a potential to be applied for real-time cytotoxicity assay.
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87.17.Ee Growth and division
33.20.Ea Infrared spectra

Real-time label-free quantitative monitoring of biomolecules without surface binding by floating-gate complementary metal-oxide semiconductor sensor array integrated with readout circuitry

Seong-Jin Kim, Kyutae Yoo, Jeoyoung Shim, Wonseok Chung, Christopher Ko, Maesoon Im, Lee-Sup Kim, and Euisik Yoon

Appl. Phys. Lett. 91, 203903 (2007); http://dx.doi.org/10.1063/1.2803848 (3 pages)

Online Publication Date: 13 November 2007

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We report a label-free field-effect sensing array integrated with complementary metal-oxide semiconductor (CMOS) readout circuitry to detect the surface potential determined by the negative charge in DNA molecules. For real-time DNA quantification, we have demonstrated the measurements of DNA molecules without immobilizing them on the sensing surface which is composed of an array of floating-gate CMOS transistors. This nonimmobilizing technique allows the continuous monitoring of the amount of charged molecules by injecting DNA solutions sequentially. We have carried out the real-time quantitative measurement of 19 bp oligonucleotides and analyzed its sensitivity as a function of pH in buffer solutions.
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87.80.-y Biophysical techniques (research methods)
87.14.G- Nucleic acids
85.30.Tv Field effect devices
87.15.N- Properties of solutions of macromolecules

Quantitative digital detection of magnetic beads using pseudo-spin-valve rings for multiplexed bioassays

J. Llandro, T. J. Hayward, D. Morecroft, J. A. C. Bland, F. J. Castaño, I. A. Colin, and C. A. Ross

Appl. Phys. Lett. 91, 203904 (2007); http://dx.doi.org/10.1063/1.2813622 (3 pages) | Cited 21 times

Online Publication Date: 15 November 2007

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We present a magnetic multiplexed assay technology which encodes the identities of target biomolecules according to the moment of magnetic beads to which they are attached. An active digital technique based on a microfabricated magnetoresistive ring-shaped sensor is demonstrated, which can distinguish the magnetic moments of micron-sized superparamagnetic beads. We propose that this development is key to combining nonvolatile magnetic labeling with biochemical libraries for high-throughput bioassays and rapid multiplexed detection.
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07.07.Df Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing
85.75.Ss Magnetic field sensors using spin polarized transport
85.70.Kh Magnetic thin film devices: magnetic heads (magnetoresistive, inductive, etc.); domain-motion devices, etc.
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