APL Nameplate
  • Volume/Page
  • Keyword
  • DOI
  • Citation
  • Advanced
   
 
 
 

Flickr Twitter UniPHY Group iResearch App Facebook

Applied Physics Letters / Volume 91 / Issue 8 / APPLIED BIOPHYSICS

Appl. Phys. Lett. 91, 083902 (2007); http://dx.doi.org/10.1063/1.2772762 (3 pages)

High density single molecule surface patterning with colloidal epitaxy

Jerrod J. Schwartz and Stephen R. Quake

Department of Bioengineering, Stanford University and Howard Hughes Medical Institute, Stanford, California 94305

View MapView Map

(Received 11 May 2007; accepted 27 July 2007; published online 21 August 2007)

Simple and inexpensive methods for dense surface patterning of single molecules will help realize the massive potential throughput of molecular arrays in biology and nanoscience. To surpass the resolvable density limit imposed by random deposition, the authors have developed a method that uses colloids to pattern single molecules at a fixed length scale. They demonstrate the ability to pattern fluorescently labeled DNA such that ∼ 38% of the available diffraction-limited regions contain exactly one molecule. This density is slightly less than the theoretical limit suggested by Monte Carlo simulations but surpasses the random deposition limit by more than threefold.

© 2007 American Institute of Physics

RELATED DATABASES

To view database links for this article, you need to log in.

KEYWORDS and PACS

Keywords

biological techniques, colloids, DNA, fluorescence, molecular biophysics, Monte Carlo methods

PACS

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.2772762

PUBLICATION DATA

ISSN

0003-6951 (print)  
1077-3118 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to citing articles, you need to log in.


Figures (3)

Access to article objects (figures, tables, multimedia) requires a subscription; log in to view available files.
(Access to supplementary files, where available, is free for this journal.)



Close

Google Calendar

ADVERTISEMENT

Your Recent History Recent History Help

Recently Viewed

  1. High density single molecule surface patterning with colloidal epitaxy
  2. Thermopile antennas for detection of millimeter waves
  3. Organic position sensitive photodetectors based on lateral donor-acceptor concentration gradients
  4. A high-reflectivity, ambient-stable graphene mirror for neutral atomic and molecular beams
  5. Transport in organic semiconductors in large electric fields: From thermal activation to field emission
Go to AIP Home
Copyright © American Institute of Physics
close