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27 Aug 2007

Volume 91, Issue 9, Articles (09xxxx)

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Appl. Phys. Lett. 91, 093110 (2007); http://dx.doi.org/10.1063/1.2775801 (3 pages)

M. Schmidbauer, Zh. M. Wang, Yu. I. Mazur, P. M. Lytvyn, G. J. Salamo, D. Grigoriev, P. Schäfer, R. Köhler, and M. Hanke
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Joining patch-clamp and atomic force microscopy techniques for studying black lipid bilayers

Erasmo Ovalle-García and Iván Ortega-Blake

Appl. Phys. Lett. 91, 093901 (2007); http://dx.doi.org/10.1063/1.2776356 (3 pages) | Cited 4 times

Online Publication Date: 28 August 2007

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An experimental protocol that enables the direct characterization of freestanding lipid bilayers through a combination of atomic force microscopy and single channel recording is presented. The method consists of producing a 15 μm diameter pore in a 3 μm thick Mylar film that delimits two vessels. The micropore was done by a glass microneedle. L-α-phosphatidylcholine bilayers were routinely painted on the pore, observed by atomic force microscopy, and tested with gramicidin D ion channels.
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87.14.Cc Lipids
87.16.D- Membranes, bilayers, and vesicles
87.64.Dz Scanning tunneling and atomic force microscopy
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