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10 Mar 2008

Volume 92, Issue 10, Articles (10xxxx)

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Appl. Phys. Lett. 92, 102101 (2008); http://dx.doi.org/10.1063/1.2890735 (3 pages)

Qing Wan, Jin Huang, Zhong Xie, Taihong Wang, Eric N. Dattoli, and Wei Lu
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Surface enhanced Raman scattering analyses of individual silver nanoaggregates on living single yeast cell wall

Athiyanathil Sujith, Tamitake Itoh, Hiroko Abe, Abdul Aziz Anas, Kenichi Yoshida, Vasudevanpillai Biju, and Mitsuru Ishikawa

Appl. Phys. Lett. 92, 103901 (2008); http://dx.doi.org/10.1063/1.2891086 (3 pages) | Cited 17 times

Online Publication Date: 10 March 2008

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We labeled the living yeast cell surface (Saccharomyces cerevisiae strain W303-1A) by silver nanoparticles which can form nanoaggregates and found to show surface enhanced Raman scattering (SERS) activity. Blinking of SERS and its polarization dependence reveal that SERS signals are from amplified electromagnetic field at nanometric Ag nanoparticles gaps with single or a few molecules sensitivity. We tentatively assigned SERS spectra from a yeast cell wall to mannoproteins. Nanoaggregate-by-nanoaggregate variations and temporal fluctuations of SERS spectra are discussed in terms of inhomogeneous mannoprotein distribution on a cell wall and possible ways of Ag nanoaggregate adsorption, respectively.
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87.17.-d Cell processes
87.80.-y Biophysical techniques (research methods)

Image reconstruction for multiphoton fluorescence microscopy

Partha Pratim Mondal, Giuseppe Vicidomini, and Alberto Diaspro

Appl. Phys. Lett. 92, 103902 (2008); http://dx.doi.org/10.1063/1.2888177 (3 pages) | Cited 12 times

Online Publication Date: 10 March 2008

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We present a general image reconstruction methodology for multiphoton fluorescence microscopy. The method is primarily based on Markov random field modeling of the detected image data. Evaluation on two-photon-excitation-(TPE) and two-photon-excited-4PI-microscopy images shows high quality artifact-free reconstruction. Image reconstruction for multiphoton imaging modalities such as TPE and 4PI may find potential application in nano-bioimaging and optical imaging.
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42.30.Wb Image reconstruction; tomography
87.64.mn Multiphoton
87.64.kv Fluorescence
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