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13 Oct 2008

Volume 93, Issue 15, Articles (15xxxx)

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Appl. Phys. Lett. 93, 151101 (2008); http://dx.doi.org/10.1063/1.2996271 (3 pages)

Xuefeng Zeng and Hongrui Jiang
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Phase retrieval for improved three-dimensional velocimetry of dynamic x-ray blood speckle

S. C. Irvine, D. M. Paganin, S. Dubsky, R. A. Lewis, and A. Fouras

Appl. Phys. Lett. 93, 153901 (2008); http://dx.doi.org/10.1063/1.3001592 (3 pages) | Cited 19 times

Online Publication Date: 15 October 2008

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High-resolution synchrotron-based x-ray phase-contrast images of in vitro blood flow have been collected. We demonstrate that the application of a single-image phase retrieval algorithm to the images leads to improved detail in particle image velocimetry correlation peaks. Out-of-plane variation in flow has been extracted from the peaks leading to the three-dimensional reconstruction of velocity across an axially symmetric cylinder.
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87.59.-e X-ray imaging
87.19.U- Hemodynamics
87.85.gf Fluid mechanics and rheology
87.57.nf Reconstruction

Bright-field analysis of phi29 DNA packaging motor using a magnetomechanical system

Chun-Li Chang, Hui Zhang, Dan Shu, Peixuan Guo, and Cagri A. Savran

Appl. Phys. Lett. 93, 153902 (2008); http://dx.doi.org/10.1063/1.3000606 (3 pages) | Cited 3 times

Online Publication Date: 17 October 2008

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We report a simple and robust magnetomechanical system for direct visual observation of the DNA packaging behavior of the bacteriophage phi29 in real time. The system comprises a micron-sized magnetic bead attached to the free end of the viral DNA, a magnet and a bright-field microscope. We show that the phi29 DNA packaging activity can be observed and dynamically analyzed at the single molecular level in bright field with a relatively simple system. With this system we also visually demonstrate the phi29 motor transporting a cargo 10 000 times the viral size.
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87.64.mc Bright field
87.15.R- Reactions and kinetics
87.80.Ek Mechanical and micromechanical techniques
85.85.+j Micro- and nano-electromechanical systems (MEMS/NEMS) and devices
87.14.gk DNA
87.15.B- Structure of biomolecules
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