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11 Aug 2008

Volume 93, Issue 6, Articles (06xxxx)

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Appl. Phys. Lett. 93, 062101 (2008); http://dx.doi.org/10.1063/1.2968206 (3 pages)

K. Shibata and K. Hirakawa
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A three-dimensional ultrasonic cage for characterization of individual cells

Otto Manneberg, Bruno Vanherberghen, Jessica Svennebring, Hans M. Hertz, Björn Önfelt, and Martin Wiklund

Appl. Phys. Lett. 93, 063901 (2008); http://dx.doi.org/10.1063/1.2971030 (3 pages) | Cited 18 times

Online Publication Date: 14 August 2008

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We demonstrate enrichment, controlled aggregation, and manipulation of microparticles and cells by an ultrasonic cage integrated in a microfluidic chip compatible with high-resolution optical microscopy. The cage is designed as a dual-frequency resonant filleted square box integrated in the fluid channel. Individual particles may be trapped three dimensionally, and the dimensionality of one-dimensional to three-dimensional aggregates can be controlled. We investigate the dependence of the shape and position of a microparticle aggregate on the actuation voltages and aggregate size, and demonstrate optical monitoring of individually trapped live cells with submicrometer resolution.
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87.85.Uv Micromanipulators
87.85.Ox Biomedical instrumentation and transducers, including micro-electro-mechanical systems (MEMS)
85.85.+j Micro- and nano-electromechanical systems (MEMS/NEMS) and devices
47.85.Np Fluidics
47.60.Dx Flows in ducts and channels
87.64.M- Optical microscopy
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