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8 Jun 2009

Volume 94, Issue 23, Articles (23xxxx)

Issue Cover Spotlight Figure

Appl. Phys. Lett. 94, 233101 (2009); http://dx.doi.org/10.1063/1.3148782 (3 pages)

E. Moyen, M. Macé, G. Agnus, A. Fleurence, T. Maroutian, F. Houzé, A. Stupakiewicz, L. Masson, B. Bartenlian, W. Wulfhekel, P. Beauvillain, and M. Hanbücken
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Facile biosynthesis of phosphate capped gold nanoparticles by a bacterial isolate Stenotrophomonas maltophilia

Yogesh Nangia, Nishima Wangoo, Saurabh Sharma, Jin-Song Wu, Vinayak Dravid, G. S. Shekhawat, and C. Raman Suri

Appl. Phys. Lett. 94, 233901 (2009); http://dx.doi.org/10.1063/1.3141519 (3 pages) | Cited 8 times

Online Publication Date: 8 June 2009

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We report intracellular biosynthesis of gold nanoparticles (GNPs) by a strain Stenotrophomonas maltophilia (AuRed02) isolated from the soil samples of Singhbhum gold mines, India. An aqueous solution of gold chloride was reduced to metallic gold in a suspension of disrupted cell mass of AuRed02, which progressively turns into cherry red within 8 h of incubation at 25 °C. The optical spectrum showed the plasmon resonance at 530 nm and analysis by transmission electron microscopy and dynamic light scattering confirmed the formation of around 40 nm GNPs. Zeta potential and Fourier transform infrared measurements confirmed GNPs are capped by negatively charged phosphate groups of NADP.
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81.07.Wx Nanopowders
81.16.-c Methods of micro- and nanofabrication and processing
81.05.Bx Metals, semimetals, and alloys
73.22.Lp Collective excitations
78.68.+m Optical properties of surfaces
78.35.+c Brillouin and Rayleigh scattering; other light scattering

Quantifying thermodynamics of collagen thermal denaturation by second harmonic generation imaging

Vladimir A. Hovhannisyan, Ping-Jung Su, Sung-Jan Lin, and Chen-Yuan Dong

Appl. Phys. Lett. 94, 233902 (2009); http://dx.doi.org/10.1063/1.3142864 (3 pages) | Cited 14 times

Online Publication Date: 8 June 2009

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Show Abstract
Time-lapse second harmonic generation (SHG) microscopy was applied for the extraction of thermodynamic parameters of collagen thermal denaturation. We found that at sufficiently high temperatures, temporal dependence of SHG intensity from the isothermal treatment of chicken dermal collagen was single exponential and can be modeled by the Arrhenius equation. Activation energy and the frequency factor of chicken dermal collagen thermal denaturation were determined using temporal decays of SHG intensity at different temperatures. Our results show that time-lapse, high temperature SHG imaging can be used to quantify kinetic properties of collagen thermal denaturation within a microscopic volume of 1 nl.
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87.19.Pp Biothermics and thermal processes in biology
87.14.E- Proteins
42.65.Ky Frequency conversion; harmonic generation, including higher-order harmonic generation
87.64.M- Optical microscopy
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