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23 Feb 2009

Volume 94, Issue 8, Articles (08xxxx)

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Appl. Phys. Lett. 94, 082501 (2009); http://dx.doi.org/10.1063/1.3085971 (3 pages)

Chunghee Nam, B. G. Ng, F. J. Castaño, M. D. Mascaro, and C. A. Ross
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Metal-enhanced bioluminescence: An approach for monitoring biological luminescent processes

Evgeni Eltzov, Daria Prilutsky, Ariel Kushmaro, Robert S. Marks, and Chris D. Geddes

Appl. Phys. Lett. 94, 083901 (2009); http://dx.doi.org/10.1063/1.3086283 (3 pages) | Cited 2 times

Online Publication Date: 23 February 2009

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In this letter, the observation of metal (plasmon)-enhanced bioluminescence is reported. Bacteria, which are capable of generating specific bioluminescence signatures upon metabolic changes (general toxicity), have been studied from both glass and silvered glass microwell bottoms, where the silvered microwells have been modified with surface deposited silver island films (SiFs). The presence of the SiFs plasmon amplifies the near-field bioluminescence signatures, ≈ <50 nm from the surface, enabling amplified detection of the reporter bioluminescence indicating sample toxicity. Using our approach a greater than fivefold enhancement in far-field bioluminescence occurs with much greater enhancements in the near-field predicted.
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87.64.kv Fluorescence
87.18.-h Biological complexity
87.15.mq Luminescence

High volume confinement in two-photon total-internal-reflection fluorescence correlation spectroscopy

D. Ivanov, V. Shcheslavskiy, I. Märki, M. Leutenegger, and T. Lasser

Appl. Phys. Lett. 94, 083902 (2009); http://dx.doi.org/10.1063/1.3077309 (3 pages) | Cited 7 times

Online Publication Date: 24 February 2009

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We report results on two-photon total-internal-reflection fluorescence correlation spectroscopy with radially polarized light. The combination of liquid crystal spatial light modulator, providing radial polarization with ultrafast laser (picosecond Nd:GdVO4 laser), allowed us to take the advantage of nonlinear optical contrast mechanisms to suppress the side-lobe energy specific for radial polarization and reduce the effective excited volume twice compared to one-photon evanescent wave excitation in fluorescence correlation spectroscopy.
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07.60.Rd Visible and ultraviolet spectrometers
42.65.Re Ultrafast processes; optical pulse generation and pulse compression

High resolution pair-distance distribution function P(r) of protein solutions

Xinguo Hong and Quan Hao

Appl. Phys. Lett. 94, 083903 (2009); http://dx.doi.org/10.1063/1.3089360 (3 pages) | Cited 1 time

Online Publication Date: 26 February 2009

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A high resolution pair-distance distribution function P(r) of protein molecules has been obtained from the complete solution scattering curve made by combining accurate small-angle and wide-angle x-ray scattering data out to 2.15 Å resolution. Both indirect and direct Fourier transforms exhibit two distinct peaks at 1.4 and 5.1 Å in P(r). X-ray crystallographic data demonstrate that these peaks correspond to two intramolecular distances: the average bond length between C, N, and O atoms and the pitch of an α-helix, respectively. Hence some high resolution aspects of the structure and function of a protein may be investigated in a solution.
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36.20.Hb Configuration (bonds, dimensions)
36.20.Ey Conformation (statistics and dynamics)
33.15.Dj Interatomic distances and angles
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