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9 Nov 2009

Volume 95, Issue 19, Articles (19xxxx)

Issue Cover Spotlight Figure

Appl. Phys. Lett. 95, 193101 (2009); http://dx.doi.org/10.1063/1.3258663 (3 pages)

L. Gaudreau, A. Kam, G. Granger, S. A. Studenikin, P. Zawadzki, and A. S. Sachrajda
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Improved conformal coatings by oblique-angle deposition for bioreplication

Drew P. Pulsifer, Akhlesh Lakhtakia, and Raúl J. Martín-Palma

Appl. Phys. Lett. 95, 193701 (2009); http://dx.doi.org/10.1063/1.3259648 (3 pages) | Cited 5 times

Online Publication Date: 9 November 2009

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The conformal-evaporated-film-by-rotation (CEFR) technique, a bioreplication method to produce high-fidelity conformal coatings on biotemplates by oblique-angle deposition, was modified to improve the uniformity of coating thickness. The substrate holding the biotemplate was rocked, in addition to rotating it about an axis passing normally through it. With the compound eyes of the common blow fly as the biotemplate, quantitative comparison of coating thickness obtained by the original CEFR and the modified CEFR techniques showed the superiority of the latter.
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87.80.-y Biophysical techniques (research methods)
81.15.Kk Vapor phase epitaxy; growth from vapor phase
68.55.at Other materials

Noncontact and noninvasive study of plant leaves using air-coupled ultrasounds

T. E. Gómez Álvarez-Arenas, D. Sancho-Knapik, J. J. Peguero-Pina, and E. Gil-Pelegrín

Appl. Phys. Lett. 95, 193702 (2009); http://dx.doi.org/10.1063/1.3263138 (3 pages) | Cited 7 times

Online Publication Date: 11 November 2009

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Plant leaves are studied by the analysis of the magnitude and phase spectra of their thickness mechanical resonances. These resonances appear at ultrasonic frequencies and have been excited and sensed using air-coupled ultrasounds. In spite of the complex leaf microstructure, the effective medium approach can be applied to solve the inverse problem, at least in the vicinity of the first thickness resonance. Results suggest that these resonances are sensitive to leaf microstructure, composition water content and water status in the leaf.
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87.80.-y Biophysical techniques (research methods)
87.50.yg Biophysical mechanisms of interaction
43.35.Yb Ultrasonic instrumentation and measurement techniques
43.80.Ev Acoustical measurement methods in biological systems and media

Label-free diagnosis of human hepatocellular carcinoma by multiphoton autofluorescence microscopy

Tzu-Lin Sun (孫梓凌), Yuan Liu (劉源), Ming-Chin Sung (宋明瑾), Hsiao-Ching Chen (陳曉菁), Chun-Hui Yang (楊淳惠), Vladimir Hovhannisyan, Wei-Chou Lin (林維洲), Wei-Liang Chen (陳維良), Ling-Ling Chiou (邱玲玲), Guan-Tarn Huang (黃冠棠), Ki Hean Kim (김기현), Peter T. C. So (蘇子正), Hsuan-Shu Lee (李宣書), and Chen-Yuan Dong (董成淵)

Appl. Phys. Lett. 95, 193703 (2009); http://dx.doi.org/10.1063/1.3250158 (3 pages) | Cited 3 times

Online Publication Date: 13 November 2009

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Conventional diagnosis for hepatocellular carcinoma (HCC) is time consuming in sample preparation and deficient in quantitative examination. In this work, we developed multiphoton autofluorescence (MAF) microscopy as an effective and efficient tool in HCC diagnosis with qualitative imaging and quantitative measurement. We found that MAF imaging effectively identifies cellular architecture in the liver specimens. Our results also demonstrate the capability of using tissue quantitative parameters of multiphoton autofluorescence intensity ratio, the nuclear number density, and nuclear-cytoplasmic ratio for tumor discrimination. This approach has the potential in clinical diagnosis of HCC and the in vivo investigation of liver tumor development in animal models.
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87.64.mn Multiphoton
87.16.-b Subcellular structure and processes
87.63.L- Visual imaging
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