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24 Aug 2009

Volume 95, Issue 8, Articles (08xxxx)

Issue Cover Spotlight Figure

Appl. Phys. Lett. 95, 082507 (2009); http://dx.doi.org/10.1063/1.3186782 (3 pages)

Sang-Koog Kim, Ki-Suk Lee, and Dong-Soo Han
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Detection of pesticide effects in human keratinocytes by means of Raman microspectroscopy

G. Perna, M. Lasalvia, A. Castro, E. Mezzenga, N. L’Abbate, P. F. Biagi, and V. Capozzi

Appl. Phys. Lett. 95, 083701 (2009); http://dx.doi.org/10.1063/1.3194143 (3 pages) | Cited 3 times

Online Publication Date: 25 August 2009

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Biochemical modifications of single cells of human keratinocytes exposed to different concentrations of chlorpyriphos solutions were detected by means of Raman microspectroscopy. The modifications mainly consist of breakdown of amide linkage between aminoacids. Fragmentation of DNA bases and single aminoacids occurs as well. The protein and DNA damage are already detectable at a chlorpyriphos concentration value (10−6M), which is at least three orders of magnitude lower than the concentration estimated as cytotoxic (10−3M). Raman microspectroscopy technique results to be a very sensitive technique to detect molecular changes even in single human cells exposed to toxic pesticide.
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87.15.R- Reactions and kinetics
33.20.Fb Raman and Rayleigh spectra (including optical scattering)
36.20.Ng Vibrational and rotational structure, infrared and Raman spectra
87.14.E- Proteins
87.14.gk DNA
87.18.-h Biological complexity

Effect of the atmospheric pressure nonequilibrium plasmas on the conformational changes of plasmid DNA

Xu Yan, Fei Zou, Xin Pei Lu, Guangyuan He, Meng Jun Shi, Qing Xiong, Xuan Gao, Zilan Xiong, Yin Li, Feng Yun Ma, Men Yu, Chang Dong Wang, Yuesheng Wang, and Guangxiao Yang

Appl. Phys. Lett. 95, 083702 (2009); http://dx.doi.org/10.1063/1.3212739 (3 pages) | Cited 11 times

Online Publication Date: 25 August 2009

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The cold atmospheric pressure plasma, which has been widely used for biomedical applications, may potentially affect the conformation of DNA. In this letter, an atmospheric pressure plasma plume is used to investigate its effects on the conformational changes of DNA of plasmid pAHC25. It is found that the plasma plume could cause plasmid DNA topology alteration, resulting in the percentage of the supercoiled plasmid DNA form decreased while that of the open circular and linearized form of plasmid DNA increased as detected by agrose gel electrophoresis. On the other hand, further investigation by using polymerase chain reaction method shows that the atmospheric pressure plasma jet treatments under proper conditions does not affect the genes of the plasmid DNA, which may have potential application in increasing the transformation frequency by genetic engineering.
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87.15.hp Conformational changes
87.14.gk DNA
87.53.-j Effects of ionizing radiation on biological systems
87.85.md Genetic engineering
52.77.-j Plasma applications
87.15.Tt Electrophoresis

Action potentials of HL-1 cells recorded with silicon nanowire transistors

Jan Felix Eschermann, Regina Stockmann, Martin Hueske, Xuan Thang Vu, Sven Ingebrandt, and Andreas Offenhäusser

Appl. Phys. Lett. 95, 083703 (2009); http://dx.doi.org/10.1063/1.3194138 (3 pages) | Cited 11 times

Online Publication Date: 27 August 2009

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Silicon nanowire (NW) transistors were fabricated in a top-down process. These devices were used to record the extracellular potential of the spontaneous activity of cardiac muscle HL-1 cells. Their signals were measured by direct dc sampling of the drain current. An improved signal-to-noise ratio compared to planar field-effect devices was observed. Furthermore the signal shape was evaluated and could be associated to different membrane currents. With these experiments, a qualitative description of the properties of the cell-NW contact was obtained and the suitability of these sensors for electrophysiological measurements in vitro was demonstrated.
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85.30.Tv Field effect devices
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