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15 Mar 2010

Volume 96, Issue 11, Articles (11xxxx)

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Appl. Phys. Lett. 96, 111901 (2010); http://dx.doi.org/10.1063/1.3358107 (3 pages)

B. Rožič, S. Krause, H. Finkelmann, G. Cordoyiannis, and Z. Kutnjak
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Label-free imaging of heme proteins with two-photon excited photothermal lens microscopy

Sijia Lu, Wei Min, Shasha Chong, Gary R. Holtom, and X. Sunney Xie

Appl. Phys. Lett. 96, 113701 (2010); http://dx.doi.org/10.1063/1.3308485 (3 pages) | Cited 16 times

Online Publication Date: 17 March 2010

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Heme proteins, such as hemoglobins and cytochromes, play important roles in various biological processes. Here we employ the two-photon excited photothermal effect as a contrast mechanism to map heme proteins distribution. Particularly, both a thermal lens scheme and a high-frequency modulation are utilized to enhance the signal-to-noise ratio. We demonstrate label-free imaging of individual red blood cells, subcellular distribution of cytochromes in live mammalian cells, and the microvascular networks in mouse ear tissue and in a zebrafish gill.
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87.64.mn Multiphoton
87.15.M- Spectra of biomolecules
87.14.E- Proteins

Spectral tagging by integrated photonic crystal resonators for highly sensitive and parallel detection in biochips

Luigi Martiradonna, Ferruccio Pisanello, Tiziana Stomeo, Antonio Qualtieri, Giuseppe Vecchio, Stefania Sabella, Roberto Cingolani, Massimo De Vittorio, and Pier Paolo Pompa

Appl. Phys. Lett. 96, 113702 (2010); http://dx.doi.org/10.1063/1.3360810 (3 pages) | Cited 5 times

Online Publication Date: 17 March 2010

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We propose a technological approach aimed at improving biochips performances, based on an efficient spectral modeling and enhancement of markers fluorescence through the insertion of photonic crystal nanocavities (PhC-NCs) in the readout area of biochips. This strategy univocally associates a specific emission wavelength to a specific bioprobe immobilized on a nanocavity, therefore guaranteeing parallel detection of multiple elements and faster analysis time. Moreover, PhC-NCs significantly enhance the markers fluorescence, thus improving the detection sensitivity.
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87.80.-y Biophysical techniques (research methods)
42.70.Qs Photonic bandgap materials

Application of thermal lens response to monitor health status of red blood cells: A quantitative study of the cell death process by extracting thermal diffusivity and size

Srivathsan Vasudevan, George Chung Kit Chen, and Marta Andika

Appl. Phys. Lett. 96, 113703 (2010); http://dx.doi.org/10.1063/1.3366723 (3 pages)

Online Publication Date: 19 March 2010

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We explore monitoring the death process of individual red blood cells (RBC) quantitatively by using thermal lens (TL) response. TL response is a noninvasive excitation/probe technique that reflects photothermal parameters (e.g., absorption, thermal diffusivity, size, etc.). Since these parameters of cells change significantly during certain biological processes, real-time TL response was performed to monitor RBC death process when incubated with ionomycin. Theoretical model developed was applied to curve-fit the TL response for extracting thermal diffusivity and size of cells. Thermal diffusivity of dying RBC is found increased by 1.7 times in comparison with healthy cell.
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87.64.K- Spectroscopy
87.85.jc Electrical, thermal, and mechanical properties of biological matter
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