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Applied Physics Letters / Volume 96 / Issue 18 / BIOPHYSICS AND BIO-INSPIRED SYSTEMS

Appl. Phys. Lett. 96, 183703 (2010); http://dx.doi.org/10.1063/1.3427394 (3 pages)

Phase diagram of 3β-[N-(N,N-dimethylaminoethane)-carbamoyl]- cholesterol−dioleoylphosphatidylethanolamine/DNA complexes suggests strategies for efficient lipoplex transfection

Daniela Pozzi1, Heinz Amenitsch2, Cristina Marchini3, and Giulio Caracciolo1

1Department of Chemistry, First Faculty of Medicine, ‘Sapienza’ University of Rome, P.le A. Moro 5, 00185 Rome, Italy
2Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences, Schmiedelstrasse 6, A-8042 Graz, Austria
3Department of Molecular Cellular and Animal Biology, University of Camerino, Via Gentile III da Varano, 62032 Camerino (MC), Italy

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(Received 26 March 2010; accepted 15 April 2010; published online 5 May 2010)

Synchrotron small angle x-ray scattering and electrophoresis on agarose gels have been applied to construct the phase diagram of the ternary complex made up of the cationic lipid 3β-[N-(N,N-dimethylaminoethane)-carbamoyl]-cholesterol, the neutral lipid dioleoylphosphatidylethanolamine and DNA. We show that nominally charge-neutral complexes coexist with free DNA, while excess cationic charge is necessary to protect all the genetic cargo. Such an extra-charge requirement diminishes as the molar fraction of neutral lipid in the bilayer increases. Furthermore, complexes with very different membrane composition and charge ratio exhibit the very same DNA protection ability. The relevance of results for transfection studies is discussed.

© 2010 American Institute of Physics

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KEYWORDS and PACS

Keywords

biochemistry, biological techniques, DNA, electrophoresis, molecular biophysics, X-ray scattering

PACS

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3427394

PUBLICATION DATA

ISSN

0003-6951 (print)  
1077-3118 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    T. Salditt, I. Koltover, J. O. Rädler, and C. R. Safinya, Phys. Rev. Lett. 79, 2582 (1997).

    C. Marchini, M. Montani, A. Amici, D. Pozzi, R. Caminiti, and G. Caracciolo, Appl. Phys. Lett. 94, 033903 (2009)APPLAB000094000003033903000001.

    G. Caracciolo, D. Pozzi, R. Caminiti, and H. Amenitsch, Appl. Phys. Lett. 87, 133901 (2005)APPLAB000087000013133901000001.


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