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31 May 2010

Volume 96, Issue 22, Articles (22xxxx)

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Appl. Phys. Lett. 96, 223101 (2010); http://dx.doi.org/10.1063/1.3439728 (3 pages)

Seokho Yun, Jeremy A. Bossard, Theresa S. Mayer, and Douglas H. Werner
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Role of nuclear tubule on the apoptosis of HeLa cells induced by femtosecond laser

Hao He, Kam Tai Chan, and Siu Kai Kong

Appl. Phys. Lett. 96, 223701 (2010); http://dx.doi.org/10.1063/1.3447365 (3 pages) | Cited 2 times

Online Publication Date: 2 June 2010

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It was found that nuclear tubules (NTs) would develop inside the nuclei of HeLa cells when they were irradiated by a femtosecond laser at the wavelength of 1554 nm for 40 s or longer. These NTs provided a pathway for the excess calcium generated by the laser to diffuse from the cytoplasm to the nucleus. Concurrently, the NTs served to spread mitochondria deep inside the nucleus so that they could initiate DNA fragmentations in regions covered by the NTs in the apoptotic cell. The role of NT as the precursor and passage to apoptosis is firmly established.
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87.16.-b Subcellular structure and processes
87.50.W- Optical/infrared radiation effects
87.14.gk DNA
42.65.Re Ultrafast processes; optical pulse generation and pulse compression
42.62.Be Biological and medical applications
87.50.wp Therapeutic applications

Real-time and indicator-free detection of aqueous nitric oxide with hydrogel film

Yu-Chiang Chao, Shih-De Yeh, Hsiao-Wen Zan, Gao-Fong Chang, Hsin-Fei Meng, Chen-Hsiung Hung, Tzu-Ching Meng, Chain-Shu Hsu, and Sheng-Fu Horng

Appl. Phys. Lett. 96, 223702 (2010); http://dx.doi.org/10.1063/1.3425895 (3 pages) | Cited 2 times

Online Publication Date: 2 June 2010

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A sensing hydrogel film is demonstrated for real-time and indicator-free detection of nitric oxide (NO) in aqueous solution. The film composed of NO probe 11,16-bisphenyl-6,6,21,21-tetramethyl-m-benzi-6,21-porphodimetheno-chloro-zinc(II) and host polymer poly(2-hydroxyethyl methacrylate). The water-containing nature of this sensing hydrogel film makes the surface area high. The response time is bellow 10 s. This sensing hydrogel film also shows high selectivity, sensitivity, and stability in various pH values.
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07.07.Df Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing
82.80.-d Chemical analysis and related physical methods of analysis
82.70.Gg Gels and sols
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