APL Nameplate
  • Volume/Page
  • Keyword
  • DOI
  • Citation
  • Advanced
   
 
 
 

Flickr Twitter UniPHY Group iResearch App Facebook

Applied Physics Letters / Volume 97 / Issue 9 / BIOPHYSICS AND BIO-INSPIRED SYSTEMS

Appl. Phys. Lett. 97, 093706 (2010); http://dx.doi.org/10.1063/1.3486681 (3 pages)

Calcium-enhanced exocytosis of gold nanoparticles

Ran Chen1, George Huang2, and Pu Chun Ke1,2

1Laboratory of Single-Molecule Biophysics and Polymer Physics, Clemson University, Clemson, South Carolina 29634, USA
2Department of Biological Sciences, Clemson University, Clemson, South Carolina 29634, USA

View MapView Map

(Received 23 July 2010; accepted 16 August 2010; published online 3 September 2010)

This paper examines the exocytosis of nanoparticles, an important but rarely documented aspect of cell responses to engineered nanomaterials. Specifically, HT-29 mammalian cells were labeled with a lipophilic Laurdan dye, and the membrane fluidity in the presence of gold nanoparticles was evaluated by the generalized polarization (GP) values derived from the fluorescence spectra of the dye. The concentration of excreted gold nanoparticles increased with increasing extracellular calcium, directly demonstrating exocytosis of nanoparticles and corroborating the inference made from the GP values of the Laurdan dye. This study provides additional information for understanding the fate of nanomaterials in biological systems.

© 2010 American Institute of Physics

RELATED DATABASES

To view database links for this article, you need to log in.

KEYWORDS and PACS

Keywords

biomedical materials, biomembrane transport, bio-optics, calcium, dyes, fluorescence, gold, light polarisation, nanobiotechnology, nanoparticles

PACS

  • 87.16.dp

    Transport, including channels, pores, and lateral diffusion

  • 87.85.Rs

    Nanotechnologies-applications

  • 87.85.J-

    Biomaterials

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3486681

PUBLICATION DATA

ISSN

0003-6951 (print)  
1077-3118 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.

For access to citing articles, you need to log in.


Figures (3)

Access to article objects (figures, tables, multimedia) requires a subscription; log in to view available files.
(Access to supplementary files, where available, is free for this journal.)



Close

Google Calendar

ADVERTISEMENT

Your Recent History Recent History Help

Recently Viewed

  1. Calcium-enhanced exocytosis of gold nanoparticles
  2. Use of square waves incident on magnetic nanoparticles to induce magnetic hyperthermia for therapeutic cancer treatment
  3. Lab-on-a-chip flow cytometer employing color-space-time coding
  4. Piezoelectric-actuated, piezoresistive-sensed circular micromembranes for label-free biosensing applications
  5. Experimental validation of the theory of wall effect on dielectrophoresis
Go to AIP Home
Copyright © American Institute of Physics
close