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27 Jun 2011

Volume 98, Issue 26, Articles (26xxxx)

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Appl. Phys. Lett. 98, 263101 (2011); http://dx.doi.org/10.1063/1.3587576 (3 pages)

Jing Ye, Yu Zhao, Libin Tang, Li-Miao Chen, C. M. Luk, S. F. Yu, S. T. Lee, and S. P. Lau
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Forward light scattering for highly sensitive detection of aggregation in crystallizing protein solutions

Takashi Wakamatsu

Appl. Phys. Lett. 98, 263701 (2011); http://dx.doi.org/10.1063/1.3603932 (3 pages) | Cited 1 time

Online Publication Date: 28 June 2011

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It is presented that a forward light scattering technique is highly sensitive to aggregation in crystallizing lysozyme solutions. Static light scattering in the crystallizing protein solutions has a characteristic radiation pattern that largely differs from that of small size silica particles dispersed in water. A method to evaluate aggregate formation was demonstrated from the dependence of forward light scattering on a precipitant concentration added to a lysozyme solution. These measurements provide possibilities for characterizing protein aggregation in crystallization process.
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87.15.nr Aggregation
87.14.ej Enzymes
87.64.Cc Scattering of visible, uv, and infrared radiation
87.15.nt Crystallization

Autoclaving as a chemical-free process to stabilize recombinant silk-elastinlike protein polymer nanofibers

Weiguo Qiu, Joseph Cappello, and Xiaoyi Wu

Appl. Phys. Lett. 98, 263702 (2011); http://dx.doi.org/10.1063/1.3604786 (3 pages) | Cited 3 times

Online Publication Date: 28 June 2011

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We report here that autoclaving is a chemical-free, physical crosslinking strategy capable of stabilizing electrospun recombinant silk-elastinlike protein (SELP) polymer nanofibers. Fourier transform infrared spectroscopy showed that the autoclaving of SELP nanofibers induced a conformational conversion of β-turns and unordered structures to ordered β-sheets. Tensile stress-strain analysis of the autoclaved SELP nanofibrous scaffolds in phosphate buffered saline at 37 °C revealed a Young’s modulus of 1.02 ± 0.28 MPa, an ultimate tensile strength of 0.34 ± 0.04 MPa, and a strain at failure of 29% ± 3%.
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87.85.Qr Nanotechnologies-design
87.15.hp Conformational changes
87.14.E- Proteins
81.16.-c Methods of micro- and nanofabrication and processing
81.05.Lg Polymers and plastics; rubber; synthetic and natural fibers; organometallic and organic materials
81.40.Gh Other heat and thermomechanical treatments

Plant-in-chip: Microfluidic system for studying root growth and pathogenic interactions in Arabidopsis

Archana Parashar and Santosh Pandey

Appl. Phys. Lett. 98, 263703 (2011); http://dx.doi.org/10.1063/1.3604788 (3 pages)

Online Publication Date: 29 June 2011

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We report a microfluidic platform for the hydroponic growth of Arabidopsis plants with high-resolution visualization of root development and root-pathogen interactions. The platform comprises a set of parallel microchannels with individual input/output ports where 1-day old germinated seedlings are initially placed. Under optimum conditions, a root system grows in each microchannel and its images are recorded over a 198-h period. Different concentrations of plant growth media show different root growth characteristics. Later, the developed roots are inoculated with two plant pathogens (nematodes and zoospores) and their physicochemical interactions with the live root systems are observed.
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87.80.Ek Mechanical and micromechanical techniques
87.15.R- Reactions and kinetics
47.85.Np Fluidics
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