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Applied Physics Letters / Volume 98 / Issue 9 / BIOPHYSICS AND BIO-INSPIRED SYSTEMS

Appl. Phys. Lett. 98, 093702 (2011); http://dx.doi.org/10.1063/1.3554415 (3 pages)

Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

Yoshihiko Arita1, Maria Leilani Torres-Mapa1, Woei Ming Lee1, Tomáš Čižmár1, Paul Campbell2, Frank J. Gunn-Moore3, and Kishan Dholakia1

1SUPA, School of Physics and Astronomy, University of St. Andrews, St. Andrews, Fife KY16 9SS, United Kingdom
2Carnegie Physics Laboratory and Division of Molecular Medicine, University of Dundee, Dundee DD1 4HN, United Kingdom
3School of Biology, Medical and Biological Sciences Building, North Haugh, University of St. Andrews, St. Andrews, Fife KY16 9TF, United Kingdom

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(Received 20 July 2010; accepted 21 December 2010; published online 2 March 2011)

We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

© 2011 American Institute of Physics

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KEYWORDS and PACS

Keywords

biological effects of laser radiation, biomembrane transport, cavitation, cellular effects of radiation, genetics, laser applications in medicine, nanomedicine, nanoparticles, spatial light modulators, surgery

PACS

  • 87.16.dp

    Transport, including channels, pores, and lateral diffusion

  • 87.85.Rs

    Nanotechnologies-applications

  • 87.50.wp

    Therapeutic applications

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3554415

PUBLICATION DATA

ISSN

0003-6951 (print)  
1077-3118 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    C. E. Bell and J. A. Landt, Appl. Phys. Lett. 10, 46 (1967)APPLAB000010000002000046000001.

    P. A. Barnes and K. E. Rieckhof, Appl. Phys. Lett. 13, 282 (1968)APPLAB000013000008000282000001.


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